{"doi":"10.3389/fimmu.2020.00418","title":"In vivo Effects of Romidepsin on T-Cell Activation, Apoptosis and Function in the BCN02 HIV-1 Kick&Kill Clinical Trial","abstract":"Romidepsin (RMD) is a well-characterized histone deacetylase inhibitor approved for the treatment of cutaneous T-cell lymphoma. In vitro and in vivo studies have demonstrated that it is able to induce HIV-1 gene expression in latently infected CD4+ T cells from HIV-1+ individuals on suppressive antiretroviral therapy. However, in vitro experiments suggested that RMD could also impair T-cell functionality, particularly of activated T cells. Thus, the usefulness of RMD in HIV-1 kick&kill strategies, that aim to enhance the immune system elimination of infected cells after inducing HIV-1 viral reactivation, may be limited. In order to address whether the in vitro observations are replicated in vivo, we determined the effects of RMD on the total and HIV-1-specific T-cell populations in longitudinal samples from the BCN02 kick&kill clinical trial (NCT02616874). BCN02 was a proof-of-concept study in 15 early treated HIV-1+ individuals that combined MVA.HIVconsv vaccination with 3 weekly infusions of RMD given as a latency-reversing-agent. Our results show that RMD induced a transient increase in the frequency of apoptotic T cells and an enhanced activation of vaccine-induced T cells. Although RMD reduced the number of vaccine-elicited T cells secreting multiple cytokines, viral suppressive capacity of CD8+ T cells was preserved over the RMD treatment. These observations have important implications for the design of effective kick&kill strategies for the HIV-1 cure.","journal":"Frontiers in Immunology","year":2020,"id":98371,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":470079,"name":"Marta Ruiz‐Riol","orcid":"0000-0003-1899-8879","position":1,"is_corresponding":false},{"id":485252,"name":"Marco A. Fernández","orcid":"0000-0002-3921-2209","position":2,"is_corresponding":false},{"id":485920,"name":"Marta Marszalek","orcid":null,"position":3,"is_corresponding":false},{"id":293558,"name":"Pep Coll","orcid":null,"position":4,"is_corresponding":false},{"id":291812,"name":"Christian Manzardo","orcid":"0000-0001-8893-6177","position":5,"is_corresponding":false},{"id":291815,"name":"Samandhy Cedeño","orcid":"0000-0001-8312-0208","position":6,"is_corresponding":false},{"id":346878,"name":"José M. Miró","orcid":"0000-0001-8057-7755","position":7,"is_corresponding":false},{"id":97919,"name":"Bonaventura Clotet","orcid":"0000-0003-3232-4598","position":8,"is_corresponding":false},{"id":291824,"name":"Tomáš Hanke","orcid":"0000-0002-6076-9546","position":9,"is_corresponding":false},{"id":291825,"name":"José Moltó","orcid":"0000-0003-4564-1963","position":10,"is_corresponding":false},{"id":97904,"name":"Beatriz Mothe","orcid":"0000-0001-9975-407X","position":11,"is_corresponding":false},{"id":97914,"name":"Christian Brander","orcid":"0000-0002-0548-5778","position":12,"is_corresponding":false},{"id":485921,"name":"the BCN02 study group","orcid":null,"position":13,"is_corresponding":false},{"id":291811,"name":"Míriam Rosás-Umbert","orcid":"0000-0002-2850-6586","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-18T22:36:34.157932Z","pmid":"32265913","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}