{"doi":"10.3389/fcvm.2022.881916","title":"Vascular Regulation by Super Enhancer-Derived LINC00607","abstract":"Vascular endothelial cells (ECs) play a pivotal role in whole body homeostasis. Recent advances have revealed enhancer-associated long non-coding RNAs (lncRNAs) as essential regulators in EC function. We investigated LINC00607, a super enhancer-derived lncRNA (SE-lncRNA) in human arteries with an emphasis on ECs. Based on public databases and our single cell RNA-sequencing (scRNA-seq) data from human arteries collected from healthy and diabetic donors, we found that LINC00607 is abundantly expressed in the arteries and its level is increased in diabetic humans. Using RNA-sequencing, we characterized the transcriptomes regulated by LINC00607 in ECs and vascular smooth muscle cells (VSMCs) and in basal and diabetic conditions in ECs. Furthermore, through transcriptomic and promoter analysis, we identified c-Myc as an upstream transcription factor of LINC00607. Finally, using scRNA-seq, we demonstrated that modified antisense oligonucleotide inhibitor of LINC00607 can reverse dysfunctional changes induced by high glucose and TNFα in ECs. Collectively, our study demonstrates a multi-pronged approach to characterize LINC00607 in vascular cells and its gene regulatory networks in ECs and VSMCs. Our findings provide new insights into the regulation and function of SE-derived lncRNAs in both vascular homeostasis and dysfunction in a cell-type and context-dependent manner, which could have a significant impact on our understanding of epigenetic regulation implicated in cardiovascular health and diseases like diabetes.","journal":"Frontiers in Cardiovascular Medicine","year":2022,"id":263259,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8671,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":297549,"name":"Yingjun Luo","orcid":"0000-0003-2271-3725","position":1,"is_corresponding":false},{"id":875619,"name":"Dongqiang Yuan","orcid":null,"position":2,"is_corresponding":false},{"id":791053,"name":"Naseeb Kaur Malhi","orcid":"0000-0002-8981-2974","position":3,"is_corresponding":false},{"id":874969,"name":"Alonso Tapia","orcid":"0000-0002-3123-9159","position":4,"is_corresponding":false},{"id":919742,"name":"Vishnu Amaram Samara","orcid":"0000-0001-5932-0461","position":5,"is_corresponding":false},{"id":283024,"name":"Rama Natarajan","orcid":"0000-0003-4494-1788","position":6,"is_corresponding":false},{"id":297552,"name":"Zhen Chen","orcid":"0000-0002-3291-1090","position":7,"is_corresponding":false},{"id":303739,"name":"Kiran Sriram","orcid":"0000-0002-7412-2380","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:26:29.321923Z","pmid":"35837599","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}