{"doi":"10.3389/fcimb.2023.1117844","title":"Development of an anti-Pseudomonas aeruginosa therapeutic monoclonal antibody WVDC-5244","abstract":"The rise of antimicrobial-resistant bacterial infections is a crucial health concern in the 21st century. In particular, antibiotic-resistant Pseudomonas aeruginosa causes difficult-to-treat infections associated with high morbidity and mortality. Unfortunately, the number of effective therapeutic interventions against antimicrobial-resistant P. aeruginosa infections continues to decline. Therefore, discovery and development of alternative treatments are necessary. Here, we present pre-clinical efficacy studies on an anti- P. aeruginosa therapeutic monoclonal antibody. Using hybridoma technology, we generated a monoclonal antibody and characterized its binding to P. aeruginosa in vitro using ELISA and fluorescence correlation spectroscopy. We also characterized its function in vitro and in vivo against P. aeruginosa . The anti- P. aeruginosa antibody (WVDC-5244) bound P. aeruginosa clinical strains of various serotypes in vitro , even in the presence of alginate exopolysaccharide. In addition, WVDC-5244 induced opsonophagocytic killing of P. aeruginosa in vitro in J774.1 murine macrophage, and complement-mediated killing. In a mouse model of acute pneumonia, prophylactic administration of WVDC-5244 resulted in an improvement of clinical disease manifestations and reduction of P. aeruginosa burden in the respiratory tract compared to the control groups. This study provides promising pre-clinical efficacy data on a new monoclonal antibody with therapeutic potential for P. aeruginosa infections.","journal":"Frontiers in Cellular and Infection Microbiology","year":2023,"id":329865,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9527,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":399396,"name":"Emel Sen-Kilic","orcid":"0000-0003-1583-2056","position":1,"is_corresponding":false},{"id":502118,"name":"Aaron C. 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Miller","orcid":"0000-0003-4888-5306","position":10,"is_corresponding":false},{"id":745875,"name":"Jason Kang","orcid":"0000-0001-5736-0005","position":11,"is_corresponding":false},{"id":399395,"name":"Catherine B. Blackwood","orcid":"0000-0002-5898-6736","position":12,"is_corresponding":false},{"id":477275,"name":"Kelly L. Weaver","orcid":"0000-0002-6954-2479","position":13,"is_corresponding":false},{"id":400833,"name":"William T. Witt","orcid":null,"position":14,"is_corresponding":false},{"id":501278,"name":"Annalisa B. Huckaby","orcid":"0000-0003-2044-1535","position":15,"is_corresponding":false},{"id":822207,"name":"Gage M. Pyles","orcid":"0000-0002-5782-5658","position":16,"is_corresponding":false},{"id":1053538,"name":"Tammy J. Clark","orcid":null,"position":17,"is_corresponding":false},{"id":1053070,"name":"Saif Al‐Qatarneh","orcid":"0000-0002-6756-1633","position":18,"is_corresponding":false},{"id":283943,"name":"George K. 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