{"doi":"10.3389/fcell.2023.1237778","title":"Visualization of c-di-GMP in multicellular Dictyostelium stages","abstract":"<jats:p>The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold <jats:italic>Dictyostelium discoideum</jats:italic> among eukaryotes. <jats:italic>Dictyostelium</jats:italic> cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores. While <jats:italic>Dictyostelium</jats:italic> is known to employ c-di-GMP to induce differentiation into stalk cells, there have been no reports of direct observation of c-di-GMP using fluorescent probes. In this study, we used a fluorescent probe used in bacteria to visualize its localization within <jats:italic>Dictyostelium</jats:italic> multicellular bodies. Cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.</jats:p>","journal":"Frontiers in Cell and Developmental Biology","year":2023,"id":688028,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1797418,"name":"Yukihisa Hayashida","orcid":null,"position":1,"is_corresponding":false},{"id":1797419,"name":"Yusuke V. 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In this study, we used a fluorescent probe used in bacteria to visualize its localization within <jats:italic>Dictyostelium</jats:italic> multicellular bodies. Cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.</jats:p>","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37547475","pmcid":"PMC10399225","openalex_id":"https://openalex.org/W4384934548","authors":[],"funders":[{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JP21K06099 JP21H05532 JP23H04082","title":null},{"funder_name":"Japan Science and Technology Agency","grant_id":"JPMJPR204B","title":null}],"total_grants":2,"fwci":0.4724,"citation_percentile":0.53660684,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.3389/fcell.2023.1237778","host_type":"journal"},{"url":"https://doi.org/10.3389/fcell.2023.1237778","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fcell.2023.1237778/full","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37547475","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10399225","host_type":"repository"},{"url":"https://doaj.org/article/96fe47f7ca5948469a60518a5818f35e","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10399225/pdf/fcell-11-1237778.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10399225","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10399225?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Slime Mold and Myxomycetes Research","Biocrusts and Microbial Ecology","Photosynthetic Processes and Mechanisms"],"mesh_terms":[],"keywords":["Multicellular organism","Dictyostelium discoideum","Slime mold","Dictyostelium","Cell biology","Stalk","Biology","Cytosol","Chemistry","Biochemistry","Cell","Gene","Enzyme","Cell differentiation","Fluorescence microscopy","Signal transduction","C-di-gmp"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T12:17:27.149193Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}