{"doi":"10.3389/fcell.2022.938709","title":"Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells","abstract":"<jats:p>Several opportunities for embryo development, stem cell maintenance, cell fate, and differentiation have emerged using induced pluripotent stem cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic stem cells (ESCs) was a challenge for many years. Here, we reprogrammed fetal fibroblasts by transient expression of the four transcription factors (Oct4, Sox2, Klf4, and c-Myc, collectively termed “OSKM” factors) and cultured in iPSC medium, supplemented with bFGF, bFGF2i, leukemia inhibitory factor (LIF), or LIF2i, and then compared these biPSC lines with bESC to evaluate the pluripotent state. biPSC lines were generated in all experimental groups. Particularly, reprogrammed cells treated with bFGF were more efficient in promoting the acquisition of pluripotency. However, LIF2i treatment did not promote continuous self-renewal. biPSCs (line 2) labeled with GFP were injected into early embryos (day 4.5) to assess the potential to contribute to chimeric blastocysts. The biPSC lines show a pluripotency state and are differentiated into three embryonic layers. Moreover, biPSCs and bESCs labeled with GFP were able to contribute to chimeric blastocysts. Additionally, biPSCs have shown promising potential for contributing to chimeric blastocysts and for future studies.</jats:p>","journal":"Frontiers in Cell and Developmental Biology","year":2022,"id":17151,"datarank":0.4529493779091976,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.08021338044099745,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.08021338044099745,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":8,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":123693,"name":"Naira Carolina Godoy Pieri","orcid":null,"position":1,"is_corresponding":false},{"id":123694,"name":"Brendon William Bessi","orcid":null,"position":2,"is_corresponding":false},{"id":123695,"name":"Lucas Simões Machado","orcid":null,"position":3,"is_corresponding":false},{"id":123696,"name":"Alessandra Bridi","orcid":null,"position":4,"is_corresponding":false},{"id":123697,"name":"Aline Fernanda de Souza","orcid":null,"position":5,"is_corresponding":false},{"id":123698,"name":"Kaiana Recchia","orcid":null,"position":6,"is_corresponding":false},{"id":123699,"name":"Paulo Fantinato Neto","orcid":null,"position":7,"is_corresponding":false},{"id":123700,"name":"Pablo Juan Ross","orcid":null,"position":8,"is_corresponding":false},{"id":123701,"name":"Fabiana Fernandes Bressan","orcid":null,"position":9,"is_corresponding":false},{"id":123702,"name":"Marcelo Fábio Gouveia Nogueira","orcid":null,"position":10,"is_corresponding":false},{"id":123692,"name":"Ramon Cesar Botigelli","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36187479","pmcid":"PMC9515551","openalex_id":"https://openalex.org/W4297900463","authors":[],"funders":[{"funder_name":"Fundação de Amparo à Pesquisa Do Estado de São Paulo","grant_id":"2012/50533-2 2015/26818-5 2016/16841-2 2013/08135-2 2019/10751-0 23038.006964/2014-43 433133/2018-0","title":null}],"total_grants":1,"fwci":0.9528,"citation_percentile":0.73462001,"influential_citations":0,"citation_trend":[{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":6}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.3389/fcell.2022.938709","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fcell.2022.938709/pdf","host_type":"GOLD"},{"url":"https://doi.org/10.3389/fcell.2022.938709","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fcell.2022.938709/full","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36187479","host_type":"repository"},{"url":"https://doaj.org/article/1fd9ee45e1bf41a0a26b854f94c62066","host_type":"repository"},{"url":"https://escholarship.org/uc/item/1w4838vc","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9515551","host_type":"repository"},{"url":"http://doi.org/10.3389/fcell.2022.938709","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9515551","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9515551?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Pluripotent Stem Cells Research","CRISPR and Genetic Engineering","Animal Genetics and Reproduction","Medicine","Biology"],"mesh_terms":[],"keywords":["SOX2","Induced pluripotent stem cell","Leukemia inhibitory factor","Embryonic stem cell","KOSR","KLF4","Biology","Cell biology","Rex1","Stem cell","Homeobox protein NANOG","Basic fibroblast growth factor","Immunology","Growth factor","Genetics","Bovine","Embryonic stem cells","Induced Pluripotent Stem Cells","Cellular Reprogramming","Pluripotency Maintenance"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T17:26:01.589123Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}