{"doi":"10.3389/fcell.2022.840894","title":"Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host","abstract":"The development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their transplantation into irradiated mice. Here, we describe the isolation of HoxB8-conditional progenitor cell lines that are unique in their ability to engraft in the naïve host in the absence of conditioning of the hematopoietic niche. Our results indicate that HoxB8-conditional progenitors engraft in a β1 integrin-dependent manner and transiently generate donor-derived mature neutrophils. Furthermore, we show that neutrophils derived in vivo from transplanted HoxB8-conditional progenitors are mobilized to the periphery and recruited to sites of inflammation in a manner that depends on the C-X-C chemokine receptor 2 and β2 integrins, the same mechanisms that have been described for recruitment of endogenous primary neutrophils. Together, our studies advance the understanding of HoxB8-conditional neutrophil progenitors and describe an innovative tool that, by virtue of its ability to engraft in the naïve host, will facilitate mechanistic in vivo experimentation on neutrophils.","journal":"Frontiers in Cell and Developmental Biology","year":2022,"id":276511,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9438,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":823675,"name":"Michael Danise","orcid":null,"position":1,"is_corresponding":false},{"id":823410,"name":"Kristina D. Hinman","orcid":"0000-0002-7344-0766","position":2,"is_corresponding":false},{"id":392731,"name":"Brittany M. Neumann","orcid":"0000-0001-9514-7627","position":3,"is_corresponding":false},{"id":823676,"name":"Renita Johnson","orcid":null,"position":4,"is_corresponding":false},{"id":392730,"name":"Zachary Wilson","orcid":"0000-0003-1482-7757","position":5,"is_corresponding":false},{"id":823677,"name":"Anna Chorzalska","orcid":null,"position":6,"is_corresponding":false},{"id":362100,"name":"Patrycja M. Dubielecka","orcid":"0000-0003-3987-0647","position":7,"is_corresponding":false},{"id":392734,"name":"Craig T. Lefort","orcid":"0000-0003-3100-877X","position":8,"is_corresponding":false},{"id":593656,"name":"Joshua T. Cohen","orcid":"0000-0002-6552-6578","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:28:30.709588Z","pmid":"35127689","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}