{"doi":"10.3389/fbioe.2022.840939","title":"Prokaryotic Collagen-Like Proteins as Novel Biomaterials","abstract":"Collagens are the major structural component in animal extracellular matrices and are critical signaling molecules in various cell-matrix interactions. Its unique triple helical structure is enabled by tripeptide Gly-X-Y repeats. Understanding of sequence requirements for animal-derived collagen led to the discovery of prokaryotic collagen-like protein in the early 2000s. These prokaryotic collagen-like proteins are structurally similar to mammalian collagens in many ways. However, unlike the challenges associated with recombinant expression of mammalian collagens, these prokaryotic collagen-like proteins can be readily expressed in E. coli and are amenable to genetic modification. In this review article, we will first discuss the properties of mammalian collagen and provide a comparative analysis of mammalian collagen and prokaryotic collagen-like proteins. We will then review the use of prokaryotic collagen-like proteins to both study the biology of conventional collagen and develop a new biomaterial platform. Finally, we will describe the application of Scl2 protein, a streptococcal collagen-like protein, in thromboresistant coating for cardiovascular devices, scaffolds for bone regeneration, chronic wound dressing and matrices for cartilage regeneration.","journal":"Frontiers in Bioengineering and Biotechnology","year":2022,"id":251679,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":271610,"name":"Ziyang Lan","orcid":"0000-0001-7935-137X","position":1,"is_corresponding":false},{"id":370089,"name":"Srishtee Arora","orcid":null,"position":2,"is_corresponding":false},{"id":895107,"name":"Mykel Green","orcid":"0000-0002-0072-3730","position":3,"is_corresponding":false},{"id":895108,"name":"Mariah S. Hahn","orcid":"0000-0001-8794-129X","position":4,"is_corresponding":false},{"id":271616,"name":"Elizabeth Cosgriff‐Hernandez","orcid":"0000-0002-4701-5600","position":5,"is_corresponding":false},{"id":364583,"name":"Magnus Höök","orcid":"0000-0003-2703-0309","position":6,"is_corresponding":false},{"id":687129,"name":"Jonathan Picker","orcid":null,"position":0,"is_corresponding":true}],"reference_count":207,"raw_metadata":null,"created_at":"2026-07-19T00:24:41.993282Z","pmid":"35372322","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}