{"doi":"10.3389/fbioe.2021.646363","title":"Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells","abstract":"<jats:p>At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.</jats:p>","journal":"Frontiers in Bioengineering and Biotechnology","year":2021,"id":615993,"datarank":0.6141516843333151,"base_score":4.0943445622221,"endowment":4.0943445622221,"self_citation_contribution":0.6141516843333151,"citation_network_contribution":0.0,"self_endowment_contribution":0.6141516843333151,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1587964,"name":"Zhenlin Fan","orcid":null,"position":1,"is_corresponding":false},{"id":711437,"name":"Yan Lin","orcid":"0000-0003-4384-8354","position":2,"is_corresponding":false},{"id":1587965,"name":"Tian-Yun Wang","orcid":null,"position":3,"is_corresponding":false},{"id":1363578,"name":"Weifeng Li","orcid":"0000-0002-0244-2908","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells","abstract":"<jats:p>At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.</jats:p>","is_dataset_classified":null,"base_score":4.0943445622221,"endowment":4.0943445622221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33791287","pmcid":"PMC8006267","openalex_id":"https://openalex.org/W3138321581","authors":[],"funders":[],"total_grants":0,"fwci":3.2567,"citation_percentile":0.93597897,"influential_citations":0,"citation_trend":[{"year":2021,"count":5},{"year":2022,"count":12},{"year":2023,"count":10},{"year":2024,"count":17},{"year":2025,"count":8},{"year":2026,"count":6}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.646363/pdf","host_type":"journal"},{"url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.646363/pdf","host_type":"publisher"},{"url":"https://www.frontiersin.org/articles/10.3389/fbioe.2021.646363/full","host_type":"publisher"},{"url":"https://doi.org/10.3389/fbioe.2021.646363","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33791287","host_type":"repository"},{"url":"https://doaj.org/article/5a36d8dc86774bbe85e235f0dddfbf5a","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8006267","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8006267","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8006267?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Viral Infectious Diseases and Gene Expression in Insects","Protein purification and stability","Virus-based gene therapy research"],"mesh_terms":[],"keywords":["Chinese hamster ovary cell","Recombinant DNA","Ovary","Yield (engineering)","Function (biology)","Hamster","Cell biology","Chemistry","Biology","Biochemistry","Molecular biology","Materials science","Gene","Endocrinology","Receptor","Cell culture","Glycosylation","Chinese Hamster Ovary Cells","Recombinant Therapeutic Protein","Serum Free Medium"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T21:39:44.625032Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}