{"doi":"10.3329/bjp.v12i3.32501","title":"Detection of cytosolic tRNA in mammal by Northern blot analysis","abstract":"<jats:p>Out of entire cascade of technologies and strategies, Northern blot assay remains the most preferential approach for immediate and accurate evaluation of expressed RNA species. However, an abundance of tRNAs species under physiological conditions compared to other small RNAs makes it difficult to accurately evaluate their transcriptional alterations through traditional Northern blot assay. Here, we describe an efficient protocol for detecting subtle alterations in tRNA species in mammals by a modified Northern blot assay. This report also compares the chemical versus UV-based crosslinking of tRNA species to the surface of solid supports.Video Clip of Methodology:Detection of cytosolic tRNA in mammal by Northern blot analysis:   16 min 55 sec   Full Screen   Alternate</jats:p>","journal":"Bangladesh Journal of Pharmacology","year":2017,"id":34223,"datarank":0.2979790023705603,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.1331871590703438,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.1331871590703438,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":177624,"name":"Hye-Young Jeong","orcid":null,"position":1,"is_corresponding":false},{"id":177625,"name":"Jong-Hun Han","orcid":null,"position":2,"is_corresponding":false},{"id":177626,"name":"Irfan Ahmad Rather","orcid":null,"position":3,"is_corresponding":false},{"id":177623,"name":"Alshammari Fanar Hamad","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18998881","pmcid":null,"openalex_id":"https://openalex.org/W2725341754","authors":[],"funders":[],"total_grants":0,"fwci":0.1121,"citation_percentile":0.50435426,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2021,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.3329/bjp.v12i3.32501","host_type":"journal"},{"url":"https://doi.org/10.3329/bjp.v12i3.32501","host_type":"GOLD"},{"url":"https://doi.org/10.3329/bjp.v12i3.32501","host_type":"publisher"},{"url":"https://www.banglajol.info/index.php/BJP/article/download/32501/22386","host_type":"publisher"},{"url":"https://doaj.org/article/dea5011ecaa140e2a3ec61e629d6b7a5","host_type":"repository"}],"fields_of_study":["RNA modifications and cancer","Cancer-related molecular mechanisms research","RNA and protein synthesis mechanisms","Biology"],"mesh_terms":[],"keywords":["Northern blot","Western blot","Transfer RNA","Dot blot","Blot","RNA","Cytosol","Biology","Molecular biology","Biochemistry","Gene","Enzyme"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T17:59:50.540731Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}