{"doi":"10.33218/001c.90447","title":"A Synergetic Approach Utilizing Nanotechnology, Chemotherapy, and Radiotherapy for Pancreatic Cancer Treatment","abstract":"<img src=” https://s3.amazonaws.com/production.scholastica/article/90447/large/prnano_1112023ga.jpg?1700670543”> The objective of this study was to assess the anticancer effectiveness of gold nanoparticles (GNPs) and lipid-encapsulated docetaxel prodrug (LNPDTX-P) with radiotherapy (RT). The study utilized a co-culture spheroid model comprising MIA PaCa-2 cancer cells and patient-derived cancer-associated fibroblasts (CAF-98) to mimic pancreatic cancer conditions. The spheroids underwent treatment with GNPs (7.5 μg/mL), LNPDTX-P (99 nM of DTX pro-drug), and 2 Gy of RT. Cell viability of the spheroids was evaluated using the CellTiter-Glo 3D assay. At the same time, DNA double-strand breaks (DSBs) were assessed by examining the expression of the DNA damage marker 53BP1 through an immunofluorescence assay. Alt-hough GNPs/RT and RT/LNPDTX-P showed a reduction in spheroid size and an apparent in-crease in DNA DSB damage, the combination of the two nanoparticles, GNPs, and LNPDTX-P, with RT, significantly enhanced the anticancer efficacy, resulting in a 28% decrease in spheroid size and an estimated 39% increase in DNA DSB. The combination of GNPs and LNPDTX-P with RT showed a synergetic effect due to their radiosensitizing properties, improving the ther-apeutic efficacy of each treatment modality alone. This triple modality offers a hopeful strategy to enhance cancer treatment efficacy while reducing adverse effects.","journal":"Precision Nanomedicine","year":2023,"id":381295,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9643,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1147266,"name":"Reinali Calisin","orcid":null,"position":1,"is_corresponding":false},{"id":986656,"name":"Nolan Jackson","orcid":"0009-0009-8341-7875","position":2,"is_corresponding":false},{"id":1067402,"name":"Jessica Morgan","orcid":"0000-0001-9741-1866","position":3,"is_corresponding":false},{"id":987134,"name":"Sam Chen","orcid":null,"position":4,"is_corresponding":false},{"id":987135,"name":"Yuen Yi C. Tam","orcid":null,"position":5,"is_corresponding":false},{"id":1068008,"name":"Wayne Beckham","orcid":null,"position":6,"is_corresponding":false},{"id":257654,"name":"Sunil Krishnan","orcid":"0000-0002-1340-4771","position":7,"is_corresponding":false},{"id":257624,"name":"Devika B. Chithrani","orcid":"0000-0001-5251-1388","position":8,"is_corresponding":false},{"id":986655,"name":"Abdulaziz Alhussan","orcid":"0000-0002-9920-2803","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T01:17:08.831117Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}