{"doi":"10.32920/22027733","title":"Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples","abstract":"<p>In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access to diagnostic tests. Here we report the results of double-blinded studies of the performance of paper-based diagnostic tests for the Zika and chikungunya viruses in a field setting in Latin America. The tests involved a cell-free expression system relying on isothermal amplification and toehold-switch reactions, a purpose-built portable reader and onboard software for computer vision-enabled image analysis. In patients suspected of infection, the accuracies and sensitivities of the tests for the Zika and chikungunya viruses were, respectively, 98.5% (95% confidence interval, 96.2-99.6%, 268 serum samples) and 98.5% (95% confidence interval, 91.7-100%, 65 serum samples) and approximately 2 aM and 5 fM (both concentrations are within clinically relevant ranges). The analytical specificities and sensitivities of the tests for cultured samples of the viruses were equivalent to those of the real-time quantitative PCR. Cell-free synthetic biology tools and companion hardware can provide de-centralized, high-capacity and low-cost diagnostics for use in low-resource settings.</p> <p>Double-blinded studies show that paper-based diagnostic tests and a companion portable device designed for use in low-resource settings perform well for the diagnosis of the Zika and chikungunya viruses in serum samples.</p>","journal":null,"year":2023,"id":402879,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.962,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":851527,"name":"Severino Jefferson Ribeiro da Silva","orcid":"0000-0002-3779-4727","position":1,"is_corresponding":false},{"id":637668,"name":"Yuxiu Guo","orcid":null,"position":2,"is_corresponding":false},{"id":637667,"name":"Seray Cicek","orcid":null,"position":3,"is_corresponding":false},{"id":851528,"name":"Larissa Krokovsky","orcid":"0009-0007-1497-5991","position":4,"is_corresponding":false},{"id":852233,"name":"Paige Homme","orcid":null,"position":5,"is_corresponding":false},{"id":851529,"name":"Yilin Xiong","orcid":"0000-0003-4048-4380","position":6,"is_corresponding":false},{"id":852234,"name":"Talia Xu","orcid":null,"position":7,"is_corresponding":false},{"id":851530,"name":"María-Angélica Calderón-Peláez","orcid":"0000-0003-0788-0795","position":8,"is_corresponding":false},{"id":852235,"name":"Sigrid Camacho-Ortega","orcid":null,"position":9,"is_corresponding":false},{"id":1168530,"name":"Duo Ma","orcid":"0009-0000-5585-6621","position":10,"is_corresponding":false},{"id":575916,"name":"Jurandy Júnior Ferraz de Magalhães","orcid":"0000-0003-2774-4627","position":11,"is_corresponding":false},{"id":851531,"name":"Bárbara Nayane Rosário Fernandes Souza","orcid":"0000-0002-6366-2533","position":12,"is_corresponding":false},{"id":851532,"name":"Diego Guerra de Albuquerque Cabral","orcid":"0000-0003-3269-9154","position":13,"is_corresponding":false},{"id":636956,"name":"Katariina Jaenes","orcid":"0000-0003-1434-6785","position":14,"is_corresponding":false},{"id":852236,"name":"Polina Sutyrina","orcid":null,"position":15,"is_corresponding":false},{"id":852237,"name":"Tom Ferrante","orcid":null,"position":16,"is_corresponding":false},{"id":851533,"name":"Andrea Denisse Benítez","orcid":"0000-0002-3599-3044","position":17,"is_corresponding":false},{"id":851534,"name":"Victoria Nipáz","orcid":"0000-0001-6769-0403","position":18,"is_corresponding":false},{"id":702591,"name":"Patricio Ponce","orcid":"0000-0002-7627-1056","position":19,"is_corresponding":false},{"id":851535,"name":"Darius G. 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