{"doi":"10.3233/jad-221118","title":"Evaluation of Blood-Based Plasma Biomarkers as Potential Markers of Amyloid Burden in Preclinical Alzheimer’s Disease","abstract":"BACKGROUND: Participant eligibility for the A4 Study was determined by amyloid PET imaging. Given the disadvantages of amyloid PET imaging in accessibility and cost, blood-based biomarkers may serve as a sufficient biomarker and more cost-effective screening tool for patient enrollment into preclinical AD trials. OBJECTIVE: To determine if a blood-based screening test can adequately identify amyloid burden in participants screened into a preclinical AD trial. METHODS: In this cross-sectional study, 224 participants from the A4 Study received an amyloid PET scan (18Florbetapir) within 90 days of blood sample collection. Blood samples from all study participants were processed within 2 h after phlebotomy. Plasma amyloid measures were quantified by Shimazdu and C2 N Diagnostics using mass spectrometry-based platforms. A corresponding subset of blood samples (n = 100) was processed within 24 h after phlebotomy and analyzed by C2 N. RESULTS: Plasma Aβ42/Aβ40 demonstrated the highest association for Aβ accumulation in the brain with an AUC 0.76 (95%CI = 0.69, 0.82) at C2 N and 0.80 (95%CI = 0.75, 0.86) at Shimadzu. Blood samples processed to plasma within 2 h after phlebotomy provided a better prediction of amyloid PET status than blood samples processed within 24 h (AUC 0.80 versus 0.64; p < 0.001). Age, sex, and APOE ɛ4 carrier status did not the diagnostic performance of plasma Aβ42/Aβ40 to predict amyloid PET positivity in A4 Study participants. CONCLUSION: Plasma Aβ42/Aβ40 may serve as a potential biomarker for predicting elevated amyloid in the brain. Utilizing blood testing over PET imaging may improve screening efficiency into clinical trials.","journal":"Journal of Alzheimer s Disease","year":2023,"id":356943,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":363438,"name":"Oliver Langford","orcid":"0009-0006-8951-1326","position":1,"is_corresponding":false},{"id":1106452,"name":"Natalie Levin","orcid":null,"position":2,"is_corresponding":false},{"id":226625,"name":"Rema Raman","orcid":"0000-0002-4096-6390","position":3,"is_corresponding":false},{"id":621688,"name":"Kevin E. Yarasheski","orcid":"0000-0001-5436-2451","position":4,"is_corresponding":false},{"id":595915,"name":"Tim West","orcid":"0000-0003-3267-8249","position":5,"is_corresponding":false},{"id":948771,"name":"Sara Abdel‐Latif","orcid":null,"position":6,"is_corresponding":false},{"id":51716,"name":"Michael Donohue","orcid":"0000-0001-6026-2238","position":7,"is_corresponding":false},{"id":668062,"name":"Akinori Nakamura","orcid":"0000-0002-4259-1297","position":8,"is_corresponding":false},{"id":1106453,"name":"Kenji Toba","orcid":null,"position":9,"is_corresponding":false},{"id":230035,"name":"Colin L. Masters","orcid":"0000-0003-3072-7940","position":10,"is_corresponding":false},{"id":236162,"name":"James D. Doecke","orcid":"0000-0003-2863-0293","position":11,"is_corresponding":false},{"id":55000,"name":"Reisa A. Sperling","orcid":"0000-0003-1535-6133","position":12,"is_corresponding":false},{"id":229340,"name":"Paul Aisen","orcid":null,"position":13,"is_corresponding":false},{"id":230262,"name":"Robert A. Rissman","orcid":"0000-0001-9245-8278","position":14,"is_corresponding":false},{"id":459243,"name":"Charisse N. Winston","orcid":"0000-0002-1966-9252","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T01:13:30.556819Z","pmid":"36710683","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}