{"doi":"10.3201/eid3008.240529","title":"Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091","abstract":"Bacterial zoonoses are established causes of severe febrile illness in East Africa. Within a fever etiology study, we applied a high-throughput 16S rRNA metagenomic assay validated for detecting bacterial zoonotic pathogens. We enrolled febrile patients admitted to 2 referral hospitals in Moshi, Tanzania, during September 2007-April 2009. Among 788 participants, median age was 20 (interquartile range 2-38) years. We performed PCR amplification of V1-V2 variable region 16S rRNA on cell pellet DNA, then metagenomic deep-sequencing and pathogenic taxonomic identification. We detected bacterial zoonotic pathogens in 10 (1.3%) samples: 3 with Rickettsia typhi, 1 R. conorii, 2 Bartonella quintana, 2 pathogenic Leptospira spp., and 1 Coxiella burnetii. One other sample had reads matching a Neoerhlichia spp. previously identified in a patient from South Africa. Our findings indicate that targeted 16S metagenomics can identify bacterial zoonotic pathogens causing severe febrile illness in humans, including potential novel agents.","journal":"Emerging infectious diseases","year":2024,"id":445145,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9103,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1157008,"name":"Sarah W. Sheldon","orcid":null,"position":1,"is_corresponding":false},{"id":1261052,"name":"Luke C. Kingry","orcid":"0000-0002-5724-2575","position":2,"is_corresponding":false},{"id":412596,"name":"Jeannine M. Petersen","orcid":null,"position":3,"is_corresponding":false},{"id":345414,"name":"Venance P. Maro","orcid":"0000-0003-3838-0312","position":4,"is_corresponding":false},{"id":781102,"name":"Grace Kinabo","orcid":null,"position":5,"is_corresponding":false},{"id":496728,"name":"Wilbrod Saganda","orcid":null,"position":6,"is_corresponding":false},{"id":372158,"name":"Michael J. Maze","orcid":"0000-0001-8909-1508","position":7,"is_corresponding":false},{"id":345422,"name":"Jo E. B. Halliday","orcid":"0000-0002-1329-9035","position":8,"is_corresponding":false},{"id":675627,"name":"William L. Nicholson","orcid":"0000-0002-2215-6837","position":9,"is_corresponding":false},{"id":495856,"name":"Renee L. Galloway","orcid":"0000-0003-4627-0906","position":10,"is_corresponding":false},{"id":345416,"name":"Matthew P. Rubach","orcid":"0000-0001-5262-1421","position":11,"is_corresponding":false},{"id":22443,"name":"John A. Crump","orcid":"0000-0002-4529-102X","position":12,"is_corresponding":false},{"id":3898,"name":"Robert Rolfe","orcid":"0000-0001-9181-4023","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:01:42.089235Z","pmid":"39043406","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}