{"doi":"10.3109/03008209609028887","title":"Proteoglycan-Degrading Activity of Human Stromelysin-1 and Leukocyte Elastase in Rabbit Joints. Quantitation of Proteoglycan and a Stromelysin-lnduced HABR Fragment of Aggrecan in Synovial Fluid and Cartilage","abstract":null,"journal":"Connective Tissue Research","year":1996,"id":630383,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1134424,"name":"Vernon L. 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Mumford","orcid":null,"position":11,"is_corresponding":false},{"id":1633071,"name":"Julie M. Olszewski","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Proteoglycan-Degrading Activity of Human Stromelysin-1 and Leukocyte Elastase in Rabbit Joints. Quantitation of Proteoglycan and a Stromelysin-lnduced HABR Fragment of Aggrecan in Synovial Fluid and Cartilage","abstract":"The objective of this study was to compare the specificity and potency of recombinant human SLN-1 (rhSLN) and human leukocyte elastase (HLE) as proteoglycan (PG)-degrading enzymes after intraarticular injection into rabbits. Another objective was to evaluate the elicitation of a rhSLN-induced hyaluronan-binding region (HABR) fragment from rabbit aggrecan in joints using a polyclonal antiserum (anti-FVDIPEN) against the synthetic peptide, Phe-Val-Asp-Ile-Pro-Glu-Asn (FVDIPEN). The intraarticular injection of either activated rhSLN or HLE resulted in enzyme-specific quantitative release of PG fragments into synovial fluid. Based on the criteria used herein, HLE appears to be a more potent PG-degrading enzyme than SLN. Intraarticular injection of rhSLN also resulted in time- and dose-dependent release of a new HABR fragment of aggrecan (HABR-FMDIPEN) into both articular cartilage and synovial fluid. HABR-FVDIPEN is likely to be a good marker of matrix metalloproteinase (MMP)-induced degradation of aggrecan.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8834447","pmcid":null,"openalex_id":"https://openalex.org/W2011991834","authors":[],"funders":[],"total_grants":0,"fwci":0.9522,"citation_percentile":0.75515464,"influential_citations":0,"citation_trend":[{"year":2020,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://www.tandfonline.com/doi/pdf/10.3109/03008209609028887","host_type":"publisher"},{"url":"https://doi.org/10.3109/03008209609028887","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8834447","host_type":"repository"}],"fields_of_study":["Osteoarthritis Treatment and Mechanisms","Protease and Inhibitor Mechanisms","Cell Adhesion Molecules Research"],"mesh_terms":["Amino Acid Sequence","Animals","Cartilage, Articular","Dose-Response Relationship, Drug","Female","Humans","Hyaluronic Acid","Joints","Molecular Sequence Data","Proteoglycans","Rabbits","Synovial Fluid","Time Factors","Extracellular Matrix Proteins","Leukocyte Elastase","Matrix Metalloproteinase 3","Lectins, C-Type","Aggrecans","Matrix Metalloproteinase Inhibitors"],"keywords":["Aggrecan","Proteoglycan","Synovial fluid","Cartilage","Chemistry","Matrix metalloproteinase","Metalloproteinase","Elastase","ADAMTS","Articular cartilage","Osteoarthritis","Anatomy","Medicine","Pathology","Biochemistry","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T21:08:15.770868Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}