{"doi":"10.31031/cjmi.2020.03.000570","title":"Comparison of Ultrabio HIV DNA PCR and Gag Real-Time PCR Assays for Total Hiv-1 DNA Quantification","abstract":"Total HIV-1 DNA has been shown to reflect reservoir size in HIV-1 infected individuals and correlate with viral rebound time after therapy interruption. Quantification of total HIV-1 DNA level may be used to study and evaluate reservoir elimination therapies. There is currently no FDA approved HIV- 1 DNA quantification assay available. The performance of a research uses only kit, Ultrabio HIV DNA quantification kit, was compared to a commonly used laboratory qPCR method targeting the gag region (GAG PCR). The limit of detection (LOD) and precision was evaluated with serially diluted 8E5/LAV DNA. LOD of Ultrabio Kit and GAG PCR was determined to be 4 and 25 copies/reaction, respectively. The Ultrabio Kit showed less variation when HIV-1 DNA concentration was lower than 20 copies/reaction. Twentysix HIV-1 infected patients’ PBMCs samples were quantified by both methods and the Ultrabio kit showed higher sensitivity. GAG PCR was unable to detect 2 of the samples while Ultrabio Kit was able to detect all. Both 2 samples had undetectable viral load. The quantified HIV DNA copies/million cells of the 24 detected samples by both methods showed good correlation, r=0.91. The difference was within one log.","journal":"Cohesive Journal of Microbiology & Infectious Disease","year":2020,"id":135029,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":590565,"name":"Can Yuan","orcid":"0009-0003-0773-4381","position":0,"is_corresponding":true}],"reference_count":29,"raw_metadata":null,"created_at":"2026-07-18T23:16:27.963294Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}