{"doi":"10.2967/jnumed.119.241059","title":"Moving Toward Multicenter Therapeutic Trials in Amyotrophic Lateral Sclerosis: Feasibility of Data Pooling Using Different Translocator Protein PET Radioligands","abstract":"Neuroinflammation has been implicated in amyotrophic lateral sclerosis (ALS) and can be visualized using translocator protein (TSPO) radioligands. To become a reliable pharmacodynamic biomarker for ALS multicenter trials, TSPO radioligands have some challenges to overcome. We aimed to investigate whether multicenter data pooling of different TSPO tracers ( 11 C-PBR28 and 18 F-DPA714) is feasible, after validation of an established 11 C-PBR28 PET pseudo reference analysis technique for 18 F-DPA714. Methods: Seven ALS patients from Belgium (58.9 6.7 y old, 5 men and 2 women), 8 healthy volunteers from Belgium (52.1 15.2 y old, 3 men and 5 women), 7 ALS patients from the United States (53.4 9.8 y old, 5 men and 2 women), and 7 healthy volunteers from the United States (54.6 9.6 y old, 4 men and 3 women) from a previously published study underwent dynamic 18 F-DPA714 (Leuven, Belgium) or 11 C-PBR28 (Boston, Massachusetts) PET/MRI. For 18 F-DPA714, maps of total volume of distribution (V T ) were compared with SUV ratio (SUVR) images from 40 to 60 min after injection (SUVR 40-60 ) calculated using the pseudo reference regions cerebellum, occipital cortex, and whole brain (WB) without ventricles. For 11 C-PBR28, SUVR images from 60 to 90 min after injection using the WB without ventricles were calculated. Results: In line with previous studies, increased 18 F-DPA714 uptake (17.0% 5.6%) in primary motor cortices was observed in ALS subjects, as measured by both V T and SUVR 40-60 approaches. The highest sensitivity was found for SUVR calculated using the WB without ventricles (average cluster, 21.6% 0.1%). 18 F-DPA714 V T ratio was highly correlated with the SUVR 40-60 (r . 0.8, P , 0.001). A similar pattern of increased uptake (average cluster, 20.5% 0.5%) in the primary motor cortices was observed in ALS subjects for 11 C-PBR28 SUVR calculated using the WB without ventricles. Analysis of the 18 F-DPA714 and 11 C-PBR28 data together resulted in a more extensive pattern of significantly increased glial activation bilaterally in the primary motor cortices. Conclusion: The same pseudo reference region analysis technique for 11 C-PBR28 PET can be extended toward 18 F-DPA714 PET. Therefore, in ALS, standardized analysis across these 2 tracers enables pooling of TSPO PET data across multiple centers and increases the power of TSPO as a biomarker for future therapeutic trials.","journal":"Journal of Nuclear Medicine","year":2020,"id":97010,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9535,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":226523,"name":"Suma Babu","orcid":"0000-0002-2648-7625","position":1,"is_corresponding":false},{"id":480491,"name":"Joke De Vocht","orcid":"0000-0003-4116-192X","position":2,"is_corresponding":false},{"id":308497,"name":"Nicole R. 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Hooker","orcid":"0000-0002-9394-7708","position":11,"is_corresponding":false},{"id":351192,"name":"Koen Van Laere","orcid":"0000-0001-5200-7245","position":12,"is_corresponding":false},{"id":262679,"name":"Nazem Atassi","orcid":"0000-0002-1856-2330","position":13,"is_corresponding":false},{"id":481776,"name":"Donatienne Van Weehaeghe","orcid":null,"position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T22:35:34.520494Z","pmid":"32169920","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}