{"doi":"10.29090/psa.2021.06.21.032","title":"Ya-hom, a Thai herbal remedy, has opposing effects on histaminergic and cholinergically mediated acid secretory related responses in gastric glands isolated from rabbits","abstract":"Ya-hom, a Thai traditional medicine, is used as a treatment for gastric discomfort. We previously found that Ya-hom inhibits both histamine and carbachol-stimulated acid secretion in gastric fistula rats. Herein, we used isolated gastric glands and highly enriched parietal cells from rabbit to determine whether or not Ya-hom acts directly on the acid-secreting parietal cell. Ya-hom inhibited carbachol-stimulated [ 14 C] aminopyrine (AP) accumulation in rabbit gastric glands and prevented carbachol-induced elevation of [Ca 2+ ]i in parietal cells. In contrast, Ya-hom potentiated histamine-stimulated AP accumulation and increased the stimulatory effect of the specific adenylyl cyclase activator, forskolin, in the presence of the histamine H2 receptor antagonist, cimetidine. Ya-hom had no effect on downstream cAMP-dependent signaling events including phosphorylation of LASP1 (LIM and SH3 domain protein 1), and F-actin redistribution. Because the cholinergic signaling pathway is mediated via calcium-dependent mechanisms, we compared effects of Ya-hom on carbachol-induced elevation of [Ca 2+ ]i with those of thapsigargin, a specific endoplasmic reticulum Ca 2+ pump inhibitor that also potentiates histamine-stimulated AP accumulation. Both agents similarly potentiated histamine-stimulated AP accumulation and their actions were not additive. Furthermore, Ya-hom inhibited thapsigargin-induced elevation of [Ca 2+ ]i. Based on these findings, we postulate that Ya-hom induces a slow release of calcium from intracellular stores thereby suppressing the cholinergic response and potentiating the cAMP signaling pathway linked to the histamine response. Since Ya-hom directly inhibits the stimulatory effect of carbachol on acid secretion, the gastric action of this herbal remedy may target vagally-dependent HCl secretion.","journal":"Pharmaceutical Sciences Asia","year":2021,"id":227409,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":831753,"name":"Hai-Yen Qin","orcid":null,"position":1,"is_corresponding":false},{"id":831754,"name":"Suwan Thirawarapan","orcid":null,"position":2,"is_corresponding":false},{"id":831755,"name":"Nuntavan Bunyapraphatsara","orcid":null,"position":3,"is_corresponding":false},{"id":831756,"name":"Cathaline Chew","orcid":null,"position":4,"is_corresponding":false},{"id":831752,"name":"Wisuda Suvitayavat","orcid":null,"position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-18T23:54:42.179886Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}