{"doi":"10.26717/bjstr.2023.53.008398","title":"\"Calcium Buffering by Fluorescent Indicators – Implications and Easy Solutions\"","abstract":"Intracellular free calcium concentrations ([Ca2+]i) are commonly measured with fluorescent indicators. As Ca2+ chelators, they compete with endogenous buffers for Ca2+ ions, which may reduce [Ca2+]i. Indicator concentrations that are unlikely to markedly affect Ca2+ signals were defined long time ago, but they are usually too low to allow accurate fluorescence measurements. In consequence, most routine [Ca2+] i measurements utilize much higher indicator concentrations and may underestimate [Ca2+]i. Here, we propose a method to derive correct, indicator-independent) [Ca2+]i by extrapolating the reported [Ca2+]i to the indicator-free environment. Although similar to its predecessors, the proposed steady-state model avoids complexities of earlier approaches and requires only data collected in AM ester loaded cells.","journal":"Biomedical Journal of Scientific & Technical Research","year":2023,"id":389150,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9428,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":699179,"name":"Krzysztof Hyrc","orcid":"0000-0002-8830-6324","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T01:18:22.323414Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}