{"doi":"10.26599/fshw.2024.9250360","title":"Reuterin-producing <i>Limosilactobacillus reuteri</i> with glycerol metabolism ameliorates <i>Staphylococcus aureus</i> -induced intestinal inflammation and dysfunction without gut microbiota dependent","abstract":"<em>Limosilactobacillus reuteri</em> is a vertebrate symbiont that is widely appreciated as being of significant ecological importance for human health. As a unique feature, <em>L</em>. <em>reuteri</em> converts glycerol to the antimicrobial compound reuterin using enzymes encoded in its propanediol-utilization operon and evolves with host-driven diversification. Reuterin-producing <em>L. reuteri</em> HLRE13 was selectively isolated from poultry previously and confirmed to inhibit the growth of <em>Staphylococcus aureus</em> <em>in vitro</em>. However, it remains unclear whether <em>L</em>. <em>reuteri</em> HLRE13 retains these antagonistic properties when ingested in specific-pathogen-free mice. Here, we investigated the ameliorative effects and potential mechanisms of action of <em>L. reuteri</em> HLRE13 in combination with glycerol on <em>S. aureus</em>-induced infection phenotypes in mice. Firstly, our results confirmed that <em>L. reuteri</em> HLRE13 effectively inhibited the intestinal colonization of <em>S. aureus </em>CMCC26003; Secondly,<em> L. reuteri</em> HLRE13 combined with glycerol could alleviate the intestinal tissues damage caused by <em>S. aureus</em> through increasing the expression of ZO-1, Occludin and MUC-2, ameliorate the intestinal systemic inflammatory response, and maintain the balance of gut microbiota by increasing the relative abundance of <em>Lactobacillus</em> and reducing the relative abundance of <em>Staphylococcus</em>. Furthermore, the colonization resistance was also found on<em> L. reuteri</em> HLRE13 combined with glycerol against <em>S. aureus</em> in pseudo germ-free mice, and they exerted the similar effects on alleviating intestinal damage and improving immune function. Combining these results, we speculate that reuterin-producing <em>L. reuteri</em> antagonize <em>S. aureus</em> in mice without the gut microbiota-dependent manner. Overall, our findings will provide a theoretical foundation for the scientific cognition of <em>L. reiteri</em> in maintaining intestinal health by producing reuterin.","journal":"Food Science and Human Wellness","year":2024,"id":506768,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9618,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1358250,"name":"Kaixiang Zou","orcid":null,"position":1,"is_corresponding":false},{"id":1357835,"name":"Xinrui Li","orcid":"0000-0003-0211-4215","position":2,"is_corresponding":false},{"id":1357836,"name":"Ke Zhu","orcid":"0009-0007-6338-4151","position":3,"is_corresponding":false},{"id":1357837,"name":"Yijuan Li","orcid":"0000-0003-2109-2947","position":4,"is_corresponding":false},{"id":1357838,"name":"Hua Wei","orcid":"0000-0003-4341-8290","position":5,"is_corresponding":false},{"id":716517,"name":"Zhihong Zhang","orcid":"0000-0002-6229-6279","position":6,"is_corresponding":false},{"id":1357834,"name":"Jinmei Li","orcid":"0000-0002-4751-9307","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:10:54.646711Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}