{"doi":"10.26599/fshw.2022.9250237","title":"Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis","abstract":"The present study reports the structural characteristics of three polysaccharide fractions (SPS-F1, SPS-F2 and SPS-F3) isolated and purified from squash. SPS-F1(Mw=12.30 kDa) and SPS-F2 (Mw=19.40 kDa) were likely to contain HG and RG-I domain of pectic polysaccharide, respectively. SPS-F2 (Mw=270.4 kDa) was mainly composed of rhamnose, galactose and arabinose. The treatment with SPS decreased body weight gain, glucose and TG levels in T2DM rats. Twenty-five differential metabolites were identified based on urinary metabolomics analysis, which are crucial to the anti-diabetic effect of SPS. The regulation of Nicotinamide N-oxide, Histamine, cis-Aconitate, Citrate, L-Malic acid, 3-(3-Hydroxyphenyl) propanoic acid and N-Acetyl-L-aspartic acid was mainly associated with energy metabolism, gut microbiota and inflammation. Study of surface plasmon resonance revealed the binding kinetics with galectin-3 (Gal-3) and fibroblast growth factor 2 (FGF2). The <em>K<sub>D</sub></em> value of SPS-F2 and SPS-F3 to Gal-3 are 4.97 × 10<sup>-3</sup> M and 1.48 × 10<sup>-3</sup> M, indicating a weak binding affinity. All three fractions showed moderate binding to FGF2 and the affinity was SPS-F3 &gt; SPS-F2 &gt; SPS-F1. Thus, the metabolomics and SPR approach were proved to be a promising tool in exploring the anti-diabetes effects of SPS and provided a deep understanding of the mechanisms.","journal":"Food Science and Human Wellness","year":2023,"id":367564,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9419,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":233177,"name":"Fuming Zhang","orcid":"0000-0003-2803-3704","position":1,"is_corresponding":false},{"id":477444,"name":"Quanhong Li","orcid":"0009-0007-0916-5644","position":2,"is_corresponding":false},{"id":1125123,"name":"Baoguo Sun","orcid":"0000-0003-4326-8237","position":3,"is_corresponding":false},{"id":1125124,"name":"Yuyu Zhang","orcid":"0000-0003-3095-3083","position":4,"is_corresponding":false},{"id":286697,"name":"Robert J. Linhardt","orcid":"0000-0003-2219-5833","position":5,"is_corresponding":false},{"id":702531,"name":"Liang Li","orcid":"0000-0001-9086-7422","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T01:15:16.306414Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}