{"doi":"10.26508/lsa.202302282","title":"Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responses","abstract":"Protein ubiquitylation regulates key biological processes including transcription. This is exemplified by the E3 ubiquitin ligase RNF12/RLIM, which controls developmental gene expression by ubiquitylating the REX1 transcription factor and is mutated in an X-linked intellectual disability disorder. However, the precise mechanisms by which ubiquitylation drives specific transcriptional responses are not known. Here, we show that RNF12 is recruited to specific genomic locations via a consensus sequence motif, which enables co-localisation with REX1 substrate at gene promoters. Surprisingly, RNF12 chromatin recruitment is achieved via a non-catalytic basic region and comprises a previously unappreciated N-terminal autoinhibitory mechanism. Furthermore, RNF12 chromatin targeting is critical for REX1 ubiquitylation and downstream RNF12-dependent gene regulation. Our results demonstrate a key role for chromatin in regulation of the RNF12-REX1 axis and provide insight into mechanisms by which protein ubiquitylation enables programming of gene expression.","journal":"Life Science Alliance","year":2024,"id":459105,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9471,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1172107,"name":"Catriona Aitken","orcid":"0000-0002-4788-9383","position":1,"is_corresponding":false},{"id":1172108,"name":"Beatrice F. Tan","orcid":"0000-0001-6362-7474","position":2,"is_corresponding":false},{"id":245513,"name":"Danielle G. May","orcid":"0000-0002-2628-4414","position":3,"is_corresponding":false},{"id":808476,"name":"Rachel J. Chrisopulos","orcid":null,"position":4,"is_corresponding":false},{"id":245516,"name":"Kyle J. Roux","orcid":"0000-0001-7530-5528","position":5,"is_corresponding":false},{"id":502863,"name":"Jeroen Demmers","orcid":"0000-0002-8757-9611","position":6,"is_corresponding":false},{"id":342237,"name":"Samuel G. Mackintosh","orcid":"0000-0002-5530-8403","position":7,"is_corresponding":false},{"id":607691,"name":"Joost Gribnau","orcid":"0000-0001-5645-4691","position":8,"is_corresponding":false},{"id":1010808,"name":"Francisco Bustos","orcid":"0000-0001-5457-4956","position":9,"is_corresponding":false},{"id":1172109,"name":"Cristina Gontan","orcid":"0000-0002-3918-0024","position":10,"is_corresponding":false},{"id":1010809,"name":"Greg M. Findlay","orcid":"0000-0002-7222-4965","position":11,"is_corresponding":false},{"id":1010807,"name":"Carmen Espejo-Serrano","orcid":"0000-0003-4127-510X","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T02:03:55.280882Z","pmid":"38199845","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}