{"doi":"10.26508/lsa.202201683","title":"Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20","abstract":"Members of the BTB-ZF transcription factor family regulate the immune system. Our laboratory identified that family member Zbtb20 contributes to the differentiation, recall responses, and metabolism of CD8 T cells. Here, we report a characterization of the transcriptional and epigenetic signatures controlled by Zbtb20 at single-cell resolution during the effector and memory phases of the CD8 T cell response. Without Zbtb20, transcriptional programs associated with memory CD8 T cell formation were up-regulated throughout the CD8 T response. A signature of open chromatin was associated with genes controlling T cell activation, consistent with the known impact on differentiation. In addition, memory CD8 T cells lacking Zbtb20 were characterized by open chromatin regions with overrepresentation of AP-1 transcription factor motifs and elevated RNA- and protein-level expressions of the corresponding AP-1 components. Finally, we describe motifs and genomic annotations from the DNA targets of Zbtb20 in CD8 T cells identified by cleavage under targets and release under nuclease (CUT&RUN). Together, these data establish the transcriptional and epigenetic networks contributing to the control of CD8 T cell responses by Zbtb20.","journal":"Life Science Alliance","year":2023,"id":365777,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9435,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":484495,"name":"Yasmin Kamal","orcid":"0000-0003-4423-7280","position":1,"is_corresponding":false},{"id":333507,"name":"Owen M. Wilkins","orcid":"0000-0002-0882-9484","position":2,"is_corresponding":false},{"id":1006780,"name":"Chenyang Li","orcid":"0000-0001-8109-9388","position":3,"is_corresponding":false},{"id":397493,"name":"Fred Kolling","orcid":"0000-0002-6178-9901","position":4,"is_corresponding":false},{"id":1007130,"name":"Heidi W. Trask","orcid":null,"position":5,"is_corresponding":false},{"id":485580,"name":"Young-Kwang Usherwood","orcid":null,"position":6,"is_corresponding":false},{"id":363,"name":"Chao Cheng","orcid":"0000-0002-5002-3417","position":7,"is_corresponding":false},{"id":308816,"name":"H. Robert Frost","orcid":"0000-0002-6794-9945","position":8,"is_corresponding":false},{"id":484496,"name":"Edward J. Usherwood","orcid":"0000-0002-5611-9700","position":9,"is_corresponding":false},{"id":484493,"name":"Nicholas K. Preiss","orcid":"0000-0002-6731-7542","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-19T01:14:50.885797Z","pmid":"37414528","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}