{"doi":"10.26508/lsa.202000887","title":"Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar system","abstract":"The Golgi apparatus (GA) in mammalian cells is pericentrosomally anchored and exhibits a stacked architecture. During infections by members of the alphavirus genus, the host cell GA is thought to give rise to distinct mobile pleomorphic vacuoles known as CPV-II (cytopathic vesicle-II) via unknown morphological steps. To dissect this, we adopted a phased electron tomography approach to image multiple overlapping volumes of a cell infected with Venezuelan equine encephalitis virus (VEEV) and complemented it with localization of a peroxidase-tagged Golgi marker. Analysis of the tomograms revealed a pattern of progressive cisternal bending into double-lamellar vesicles as a central process underpinning the biogenesis and the morphological complexity of this vacuolar system. Here, we propose a model for the conversion of the GA to CPV-II that reveals a unique pathway of intracellular virus envelopment. Our results have implications for alphavirus-induced displacement of Golgi cisternae to the plasma membrane to aid viral egress operating late in the infection cycle.","journal":"Life Science Alliance","year":2022,"id":287693,"datarank":0.2196523067407473,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.01170815257276366,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.01170815257276366,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9622,"is_data_producer":true,"deposit_databanks":{"PDB":["3J0C"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":490280,"name":"Elaine M. Mihelc","orcid":"0000-0002-9228-8341","position":1,"is_corresponding":false},{"id":586382,"name":"Stephanie Angel","orcid":null,"position":2,"is_corresponding":false},{"id":491239,"name":"Jason Lanman","orcid":null,"position":3,"is_corresponding":false},{"id":314297,"name":"Richard Kühn","orcid":"0000-0003-4148-1026","position":4,"is_corresponding":false},{"id":271705,"name":"Robert V. Stahelin","orcid":"0000-0001-5443-7863","position":5,"is_corresponding":false},{"id":271702,"name":"Ranjan Sengupta","orcid":"0000-0003-3160-5312","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:30:01.051685Z","pmid":"36137747","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}