{"doi":"10.26508/lsa.202000840","title":"Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells","abstract":"Highly sensitive approaches to target insulin-expressing cells would allow more effective imaging, sorting, and analysis of pancreatic β-cells. Here, we introduce the use of a reaction-based probe, diacetylated Zinpyr1 (DA-ZP1), to image pancreatic β-cells and β-like cells derived from human pluripotent stem cells. We harness the high intracellular zinc concentration of β-cells to induce a fluorescence signal in cells after administration of DA-ZP1. Given its specificity and rapid uptake by cells, we used DA-ZP1 to purify live stem cell-derived β-like cells as confirmed by immunostaining analysis. We tested the ability of DA-ZP1 to image transplanted human islet grafts and endogenous mouse pancreatic islets in vivo after its systemic administration into mice. Thus, DA-ZP1 enables purification of insulin-secreting β-like cells for downstream applications, such as functional studies, gene-expression, and cell-cell interaction analyses and can be used to label engrafted human islets and endogenous mouse islets in vivo.","journal":"Life Science Alliance","year":2021,"id":188845,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":750706,"name":"Debasish Manna","orcid":"0000-0003-4446-7318","position":1,"is_corresponding":false},{"id":269958,"name":"Ercument Dirice","orcid":"0000-0003-2792-7204","position":2,"is_corresponding":false},{"id":750707,"name":"Basudeb Maji","orcid":"0000-0002-3885-5704","position":3,"is_corresponding":false},{"id":516295,"name":"Jonnell C. Small","orcid":"0000-0002-2335-6873","position":4,"is_corresponding":false},{"id":349799,"name":"Bridget K. Wagner","orcid":"0000-0002-2629-361X","position":5,"is_corresponding":false},{"id":275621,"name":"Amit Choudhary","orcid":"0000-0002-8437-0150","position":6,"is_corresponding":false},{"id":269959,"name":"Rohit Kulkarni","orcid":"0000-0001-5029-6119","position":7,"is_corresponding":false},{"id":530588,"name":"Sevim Kahraman","orcid":"0000-0002-2880-6589","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T23:49:14.547082Z","pmid":"33514654","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}