{"doi":"10.26508/lsa.202000659","title":"Dynamic changes in RNA–protein interactions and RNA secondary structure in mammalian erythropoiesis","abstract":"Two features of eukaryotic RNA molecules that regulate their post-transcriptional fates are RNA secondary structure and RNA-binding protein (RBP) interaction sites. However, a comprehensive global overview of the dynamic nature of these sequence features during erythropoiesis has never been obtained. Here, we use our ribonuclease-mediated structure and RBP-binding site mapping approach to reveal the global landscape of RNA secondary structure and RBP-RNA interaction sites and the dynamics of these features during this important developmental process. We identify dynamic patterns of RNA secondary structure and RBP binding throughout the process and determine a set of corresponding protein-bound sequence motifs along with their dynamic structural and RBP-binding contexts. Finally, using these dynamically bound sequences, we identify a number of RBPs that have known and putative key functions in post-transcriptional regulation during mammalian erythropoiesis. In total, this global analysis reveals new post-transcriptional regulators of mammalian blood cell development.","journal":"Life Science Alliance","year":2021,"id":201466,"datarank":0.3688753602821135,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.05695912903013807,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.05695912903013807,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":6,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9384,"is_data_producer":true,"deposit_databanks":{"GENBANK":["NM_001278161","NM_016956","NM_008218","NM_001083955","NM_001201391","NM_010405"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":724317,"name":"Xinjun Ji","orcid":"0000-0002-1539-2807","position":1,"is_corresponding":false},{"id":282670,"name":"Kevin A. Janssen","orcid":"0000-0002-6980-9575","position":2,"is_corresponding":false},{"id":780291,"name":"Ian M. Silverman","orcid":"0000-0003-3819-6726","position":3,"is_corresponding":false},{"id":724318,"name":"Jesse Humenik","orcid":"0000-0002-7658-9988","position":4,"is_corresponding":false},{"id":780957,"name":"Ben Garcia","orcid":null,"position":5,"is_corresponding":false},{"id":285789,"name":"Stephen A. Liebhaber","orcid":"0000-0003-3324-4485","position":6,"is_corresponding":false},{"id":295584,"name":"Brian D. Gregory","orcid":"0000-0001-7532-0138","position":7,"is_corresponding":false},{"id":780290,"name":"Mengge Shan","orcid":"0000-0003-0631-9189","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-18T23:50:56.394488Z","pmid":"34315813","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}