{"doi":"10.2337/diab.33.2.192","title":"Evidence for Suppression of Hepatic Glucose-6-Phosphatase with Carbohydrate Feeding","abstract":"<jats:p>The mechanism by which exogenous glucose stimulates the incorporation of hepatic glucose-6-phosphate into glycogen in fasted rats has not been clearly delineated. We gave glucose intragastrically over a 3.5-h period during which liver glycogen was deposited at linear rates. Simultaneous primed continuous infusion of [2-3H] or [3-3H]glucose established that under these conditions absolute carbon flow through hepatic glucose-6-phosphatase was greatly suppressed.</jats:p>\n               <jats:p>After 1 h, hepatic [UDP-glucose] and [glucose-6-phosphate] had fallen by 50–60% and the former remained low throughout the experiment. By contrast, [glucose-6-phosphate] rebounded to its initial value by 2 h and remained at this level during the subsequent hour.</jats:p>\n               <jats:p>We interpret the data as follows. Exogenous glucose, in addition to acting as a precursor of liver glucose-6-phosphate, causes diversion of the latter away from free glucose formation and into glycogen synthesis. The fall in [UDP-glucose] is in accord with a glucose-induced activation of glycogen synthase, as proposed by Hers (Annu. Rev. Biochem. 1976; 45:167–89.). However, the fall-rise sequence of glucose-6-phosphate concentration constitutes the first direct evidence in vivo for simultaneous inhibition at the level of glucose-6-phosphatase.</jats:p>","journal":"Diabetes","year":1984,"id":673635,"datarank":0.7009243251692859,"base_score":4.672828834461906,"endowment":4.672828834461906,"self_citation_contribution":0.7009243251692859,"citation_network_contribution":0.0,"self_endowment_contribution":0.7009243251692859,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":106,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1759993,"name":"Daniel W Foster","orcid":null,"position":1,"is_corresponding":false},{"id":1759994,"name":"J Denis McGarry","orcid":null,"position":2,"is_corresponding":false},{"id":1759992,"name":"Christopher B Newgard","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Evidence for Suppression of Hepatic Glucose-6-Phosphatase with Carbohydrate Feeding","abstract":"<jats:p>The mechanism by which exogenous glucose stimulates the incorporation of hepatic glucose-6-phosphate into glycogen in fasted rats has not been clearly delineated. We gave glucose intragastrically over a 3.5-h period during which liver glycogen was deposited at linear rates. Simultaneous primed continuous infusion of [2-3H] or [3-3H]glucose established that under these conditions absolute carbon flow through hepatic glucose-6-phosphatase was greatly suppressed.</jats:p>\n               <jats:p>After 1 h, hepatic [UDP-glucose] and [glucose-6-phosphate] had fallen by 50–60% and the former remained low throughout the experiment. By contrast, [glucose-6-phosphate] rebounded to its initial value by 2 h and remained at this level during the subsequent hour.</jats:p>\n               <jats:p>We interpret the data as follows. Exogenous glucose, in addition to acting as a precursor of liver glucose-6-phosphate, causes diversion of the latter away from free glucose formation and into glycogen synthesis. The fall in [UDP-glucose] is in accord with a glucose-induced activation of glycogen synthase, as proposed by Hers (Annu. Rev. Biochem. 1976; 45:167–89.). However, the fall-rise sequence of glucose-6-phosphate concentration constitutes the first direct evidence in vivo for simultaneous inhibition at the level of glucose-6-phosphatase.</jats:p>","is_dataset_classified":null,"base_score":4.672828834461906,"endowment":4.672828834461906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6319214","pmcid":null,"openalex_id":"https://openalex.org/W2134212766","authors":[],"funders":[{"funder_name":"NIADDK NIH HHS","grant_id":"AM 18573","title":null}],"total_grants":1,"fwci":13.7261,"citation_percentile":0.99289964,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2018,"count":1},{"year":2019,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://diabetesjournals.org/diabetes/article-pdf/33/2/192/352910/33-2-192.pdf","host_type":"journal"},{"url":"https://diabetesjournals.org/diabetes/article-pdf/33/2/192/352910/33-2-192.pdf","host_type":"publisher"},{"url":"https://journals.org/diabetes/diabetes/article-pdf/33/2/192/352910/33-2-192.pdf","host_type":"publisher"},{"url":"https://doi.org/10.2337/diab.33.2.192","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6319214","host_type":"repository"}],"fields_of_study":["Glycogen Storage Diseases and Myoclonus","Amino Acid Enzymes and Metabolism","Metabolism and Genetic Disorders","Animals","Blood Glucose","Dietary Carbohydrates","Glucose","Glucose-6-Phosphatase","Liver","Liver Glycogen","Male","Rats","Rats, Inbred Strains"],"mesh_terms":["Animals","Blood Glucose","Dietary Carbohydrates","Glucose","Glucose-6-Phosphatase","Liver","Liver Glycogen","Male","Rats, Inbred Strains","Rats"],"keywords":["Internal medicine","Glycogen","Endocrinology","Glucose 6-phosphatase","Glucose 6-phosphate","Glycogen synthase","Glycogen branching enzyme","Carbohydrate","Phosphatase","Glycogenesis","Chemistry","Phosphate","Carbohydrate metabolism","Biology","Biochemistry","Enzyme","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:04:03.291343Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}