{"doi":"10.2337/db20-0517","title":"Protection of Pancreatic Islets Using Theranostic Silencing Nanoparticles in a Baboon Model of Islet Transplantation","abstract":"The long-term success of pancreatic islet transplantation (Tx) as a cure for type 1 diabetes remains limited. Islet loss after Tx related to apoptosis, inflammation, and other factors continues to limit Tx efficacy. In this project, we demonstrate a novel approach aimed at protecting islets before Tx in nonhuman primates (NHPs) (baboons) by silencing a gene (caspase-3) responsible for induction of apoptosis. This was done using siRNA (siCas-3) conjugated to magnetic nanoparticles (MNs). In addition to serving as carriers for siCas-3, these nanoparticles also act as reporters for MRI, so islets labeled with MN-siCas-3 can be monitored in vivo after Tx. In vitro studies showed the antiapoptotic effect of MN-siCas-3 on islets in culture, resulting in minimal islet loss. For in vivo studies, donor baboon islets were labeled with MN-siCas-3 and infused into recipient diabetic subjects. A dramatic reduction in insulin requirements was observed in animals transplanted with even a marginal number of labeled islets compared with controls. By demonstrating the protective effect of MN-siCas-3 in the challenging NHP model, this study proposes a novel strategy to minimize the number of donor islets required from either cadaveric or living donors.","journal":"Diabetes","year":2020,"id":79666,"datarank":0.5434138792944032,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.11843187768597074,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.11843187768597074,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":8,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9527,"is_data_producer":true,"deposit_databanks":{"figshare":["10.2337/figshare.12857708"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":342940,"name":"Ping Wang","orcid":"0000-0001-6712-8939","position":1,"is_corresponding":false},{"id":413741,"name":"Kazuhiro Takeuchi","orcid":"0000-0002-5069-5694","position":2,"is_corresponding":false},{"id":413742,"name":"Katsunori Miyake","orcid":"0000-0001-8356-9646","position":3,"is_corresponding":false},{"id":414889,"name":"Yuichi Ariyoshi","orcid":null,"position":4,"is_corresponding":false},{"id":414890,"name":"Hironosuke Watanabe","orcid":null,"position":5,"is_corresponding":false},{"id":413743,"name":"Xiaojuan Chen","orcid":"0000-0002-2109-1277","position":6,"is_corresponding":false},{"id":413744,"name":"Akira Shimizu","orcid":"0000-0002-4364-9251","position":7,"is_corresponding":false},{"id":413745,"name":"Neil Robertson","orcid":"0000-0003-1691-5531","position":8,"is_corresponding":false},{"id":413746,"name":"Kazuhiko Yamada","orcid":"0000-0002-0510-5843","position":9,"is_corresponding":false},{"id":413747,"name":"Anna Moore","orcid":"0000-0002-5218-7204","position":10,"is_corresponding":false},{"id":413740,"name":"Thomas Pomposelli","orcid":"0000-0003-1144-9922","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T21:50:57.971597Z","pmid":"32855170","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}