{"doi":"10.2337/db07-0191","title":"Normal Relationship of β- and Non–β-Cells Not Needed for Successful Islet Transplantation","abstract":"<jats:p>Islets are composed mostly of β-cells, and therefore stem cell research has concentrated on generating purified β-cells, neglecting the other endocrine cell types in the islet. We investigated the presence of endocrine non–β-cells after islet transplantation. In addition, we studied whether the transplantation of pure β-cells, in volumes similar to that used in islet transplantation, would suffice to reverse hyperglycemia in diabetic mice. Rat islets were dispersed and β-cells were purified by fluorescence-activated cell sorting according to their endogenous fluorescence. After reaggregation, 600 islet equivalents of the purified β-cell aggregates were implanted into diabetic SCID mice. In mice implanted with β-cell–enriched aggregates, the hyperglycemia was reversed and good graft function over a 12-week period was observed with regard to glucose and insulin levels, glucose tolerance tests, and graft insulin content. The endocrine cell composition of the β-cell–enriched aggregates remained constant; before and 12 weeks after transplantation, the β-cell–enriched aggregates comprised 95% β-cells and 5% endocrine non–β-cells. However, islet grafts, despite originally having comprised 75% β-cells and 25% endocrine non–β-cells, comprised just 5% endocrine non–β-cells after transplantation, indicating a loss of these cells. β-Cell–enriched aggregates can effectively reverse hyperglycemia in mice, and transplanted intact islets are depleted in non–β-cells. It is therefore likely that islet non–β-cells are not essential for successful islet transplantation.</jats:p>","journal":"Diabetes","year":2007,"id":49486,"datarank":1.858067234434822,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":1.2542644808245493,"self_endowment_contribution":0.6038027536102726,"citer_contribution":1.2542644808245493,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":37,"citers_with_citation_signal":29,"citers_with_endowment":29,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":239436,"name":"Justin R. Fernandes","orcid":null,"position":1,"is_corresponding":false},{"id":239437,"name":"Jennifer Hollister-Lock","orcid":null,"position":2,"is_corresponding":false},{"id":239438,"name":"Cameron E. Nienaber","orcid":null,"position":3,"is_corresponding":false},{"id":239439,"name":"Susan Bonner-Weir","orcid":null,"position":4,"is_corresponding":false},{"id":239440,"name":"Gordon C. Weir","orcid":null,"position":5,"is_corresponding":false},{"id":239435,"name":"Aileen J.F. King","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Normal Relationship of β- and Non–β-Cells Not Needed for Successful Islet Transplantation","abstract":"<jats:p>Islets are composed mostly of β-cells, and therefore stem cell research has concentrated on generating purified β-cells, neglecting the other endocrine cell types in the islet. We investigated the presence of endocrine non–β-cells after islet transplantation. In addition, we studied whether the transplantation of pure β-cells, in volumes similar to that used in islet transplantation, would suffice to reverse hyperglycemia in diabetic mice. Rat islets were dispersed and β-cells were purified by fluorescence-activated cell sorting according to their endogenous fluorescence. After reaggregation, 600 islet equivalents of the purified β-cell aggregates were implanted into diabetic SCID mice. In mice implanted with β-cell–enriched aggregates, the hyperglycemia was reversed and good graft function over a 12-week period was observed with regard to glucose and insulin levels, glucose tolerance tests, and graft insulin content. The endocrine cell composition of the β-cell–enriched aggregates remained constant; before and 12 weeks after transplantation, the β-cell–enriched aggregates comprised 95% β-cells and 5% endocrine non–β-cells. However, islet grafts, despite originally having comprised 75% β-cells and 25% endocrine non–β-cells, comprised just 5% endocrine non–β-cells after transplantation, indicating a loss of these cells. β-Cell–enriched aggregates can effectively reverse hyperglycemia in mice, and transplanted intact islets are depleted in non–β-cells. It is therefore likely that islet non–β-cells are not essential for successful islet transplantation.</jats:p>","is_dataset_classified":null,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17563059","pmcid":null,"openalex_id":"https://openalex.org/W2160028030","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"DK-36836","title":null}],"total_grants":1,"fwci":5.5076,"citation_percentile":0.95996506,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":4},{"year":2014,"count":4},{"year":2015,"count":3},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":4},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":3}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://diabetesjournals.org/diabetes/article-pdf/56/9/2312/388910/zdb00907002312.pdf","host_type":"journal"},{"url":"https://diabetesjournals.org/diabetes/article-pdf/56/9/2312/388910/zdb00907002312.pdf","host_type":"BRONZE"},{"url":"https://diabetesjournals.org/diabetes/article-pdf/56/9/2312/388910/zdb00907002312.pdf","host_type":"publisher"},{"url":"https://journals.org/diabetes/diabetes/article-pdf/56/9/2312/388910/zdb00907002312.pdf","host_type":"publisher"},{"url":"https://doi.org/10.2337/db07-0191","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17563059","host_type":"repository"},{"url":"https://kclpure.kcl.ac.uk/portal/en/publications/da6f5975-6b7d-414e-802a-0e472ee8c443","host_type":"repository"}],"fields_of_study":["Pancreatic function and diabetes","Diabetes Management and Research","Diabetes and associated disorders","Biology","Medicine","Animals","Blood Glucose","Cell Aggregation","Cell Separation","Diabetes Mellitus, Experimental","Insulin-Secreting Cells","Islets of Langerhans","Islets of Langerhans Transplantation","Male","Mice","Mice, Inbred ICR","Rats","Rats, Sprague-Dawley","Transplantation, Heterologous","Treatment Outcome"],"mesh_terms":["Animals","Blood Glucose","Cell Aggregation","Cell Separation","Diabetes Mellitus, Experimental","Islets of Langerhans","Male","Mice, Inbred ICR","Transplantation, Heterologous","Islets of Langerhans Transplantation","Treatment Outcome","Rats, Sprague-Dawley","Insulin-Secreting Cells","Mice","Rats"],"keywords":["Islet","Enteroendocrine cell","Transplantation","Cell sorting","Cell","Endocrine system","Internal medicine","Endocrinology","Islet cell transplantation","Biology","Diabetes mellitus","Medicine","Hormone","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T20:36:57.613065Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}