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To quantify the intrinsic lifetime of deeply embedded fluorophores, we performed phantom experiments to investigate the contribution of photon migration effects on observed lifetime by calculating the fluorescence intensity decay time. A previously proposed theoretical model of migration, based on random walk theory, is also substantiated by new experimental data. The developed experimental system has been used for in vivo mouse imaging with Alexa Fluor 750 contrast agent conjugated to tumor-specific antibodies (trastuzumab [Herceptin]). Three-dimensional mapping of the fluorescence lifetime indicates lower lifetime values in superficial breast cancer tumors in mice.</jats:p>","journal":"Molecular Imaging","year":2007,"id":604866,"datarank":4.860383472624141,"base_score":4.605170185988092,"endowment":4.605170185988092,"self_citation_contribution":0.6907755278982138,"citation_network_contribution":4.169607944725928,"self_endowment_contribution":0.6907755278982138,"citer_contribution":4.169607944725928,"corpus_percentile":null,"corpus_rank":null,"citation_count":99,"citer_count":78,"citers_with_citation_signal":67,"citers_with_endowment":67,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1552049,"name":"Jason Riley","orcid":null,"position":1,"is_corresponding":false},{"id":1552051,"name":"Victor Chernomordik","orcid":null,"position":2,"is_corresponding":false},{"id":175119,"name":"Paul Smith","orcid":null,"position":3,"is_corresponding":false},{"id":294359,"name":"Randall Pursley","orcid":"0000-0002-7933-3291","position":4,"is_corresponding":false},{"id":1552052,"name":"Sang Bong Lee","orcid":null,"position":5,"is_corresponding":false},{"id":514906,"name":"Jacek Capala","orcid":"0000-0001-7631-0310","position":6,"is_corresponding":false},{"id":1552053,"name":"Gandjbakhche Amir H","orcid":null,"position":7,"is_corresponding":false},{"id":1184286,"name":"Moinuddin Hassan","orcid":"0000-0001-6466-7072","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Fluorescence Lifetime Imaging System for in Vivo Studies","abstract":"<jats:p>In this article, a fluorescence lifetime imaging system for small animals is presented. 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