{"doi":"10.2310/7290.2006.00003","title":"High-throughput Identification of Phage-derived Imaging Agents","abstract":"<jats:p>The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.</jats:p>","journal":"Molecular Imaging","year":2006,"id":639358,"datarank":0.8580887041735421,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.4422003958375749,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.4422003958375749,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":12,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":6461,"name":"Paul A. 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We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.</jats:p>","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16779967","pmcid":null,"openalex_id":"https://openalex.org/W2097320739","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"P50 CA86355","title":null}],"total_grants":1,"fwci":2.1629,"citation_percentile":0.86546662,"influential_citations":1,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":1},{"year":2021,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.2310/7290.2006.00003","host_type":"journal"},{"url":"https://journals.sagepub.com/doi/pdf/10.2310/7290.2006.00003","host_type":"GOLD"},{"url":"https://journals.sagepub.com/doi/pdf/10.2310/7290.2006.00003","host_type":"publisher"},{"url":"https://journals.sagepub.com/doi/full-xml/10.2310/7290.2006.00003","host_type":"publisher"},{"url":"https://doi.org/10.2310/7290.2006.00003","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16779967","host_type":"repository"},{"url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:41384396","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.856.9619","host_type":""},{"url":"https://doaj.org/article/5ff7768cefa444f38d2ae8947658f792","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Advanced Biosensing Techniques and Applications","Glycosylation and Glycoproteins Research","Computer Science","Medicine","Biology","Chemistry"],"mesh_terms":["Affinity Labels","Bacteriophages","Enzyme-Linked Immunosorbent Assay","Humans","Immunohistochemistry","Lipopolysaccharides","Macrophages","Microscopy, Fluorescence","Monocytes","Peptides","Protein Binding","Peptide Library","U937 Cells"],"keywords":["Phage display","High-throughput screening","Computational biology","Prioritization","Identification (biology)","Peptide library","Throughput","Small molecule","Peptide","Computer science","Biology","Chemistry","Bioinformatics","Biochemistry","Peptide sequence","Engineering","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T00:08:19.560588Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}