{"doi":"10.2307/3578073","title":"Maintenance of Intracellular Free Ca 2+ Homeostasis Following Lethal Heat Shock","abstract":null,"journal":"Radiation Research","year":1991,"id":590100,"datarank":0.49096010976970916,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.22219618938550087,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.22219618938550087,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":4,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1509872,"name":"Charles A. Vidair","orcid":null,"position":1,"is_corresponding":false},{"id":1509874,"name":"William C. Dewey","orcid":null,"position":2,"is_corresponding":false},{"id":1237614,"name":"Zhenhua Wang","orcid":"0000-0002-7980-1825","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Maintenance of Intracellular Free Ca 2+ Homeostasis Following Lethal Heat Shock","abstract":"We have manipulated the extracellular Ca2+ concentration (1.8 mM in normal Dulbecco's modified Eagle's medium) to test whether the resulting effect on intracellular free Ca2+ homeostasis was similar in heat-sterilized and nonheated mouse NIH-3T3 cells. The responsiveness of the intracellular free Ca2+ concentration to changes in the extracellular Ca2+ concentration was not affected by prior treatment of the cells with trypsin, or by the extracellular Ca2+ concentration during dye loading (indo-1, AM). Rather, the intracellular free Ca2+ concentration was dependent upon the ambient Ca2+ concentration during analysis by flow cytometry. When the extracellular Ca2+ concentration was decreased to 0.017 mM, either before or after a lethal heat shock, the intracellular free Ca2+ concentration (approximately 300 nM) decreased to a similar extent in both heated and control cells (to approximately 30-100 nM). Similarly, when the extracellular Ca2+ concentration was increased to 15.0 mM, either before or after a lethal heat shock, the intracellular free Ca2+ concentration exhibited a quantitatively similar increase in both heated and nonheated cells (to approximately 400-1000 nM). These data indicate that a lethal heat dose does not inhibit the intact cell's ability to maintain intracellular free Ca2+ homeostasis.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1924720","pmcid":null,"openalex_id":"https://openalex.org/W1979534999","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA 31813","title":null}],"total_grants":1,"fwci":0.2826,"citation_percentile":0.50030171,"influential_citations":0,"citation_trend":[{"year":2023,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.2307/3578073","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1924720","host_type":"repository"}],"fields_of_study":["Heat shock proteins research","Physiological and biochemical adaptations","Meat and Animal Product Quality"],"mesh_terms":["Animals","Calcium","Hot Temperature","Homeostasis","Shock","Time Factors","3T3 Cells","Cell Death","Mice"],"keywords":["Extracellular","Intracellular","Homeostasis","Biophysics","Chemistry","Extracellular fluid","Biochemistry","Cell biology","Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T13:07:55.727215Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}