{"doi":"10.2298/abs151015056k","title":"Subchronic exposure to acrylamide affects colon mucin secretion in juvenile wistar rats","abstract":"<jats:p>Acrylamide (AA) is an important industrial chemical worldwide. AA also forms\n   naturally in many high-carbohydrate foods (bread, French fries, coffee, etc.)\n   when they are heated. Since AA is ubiquitous in the human diet, and more than\n   one-third of the calories we take in each day come from foods with detectable\n   levels of acrylamide, the aim of this study was to determine the effect of\n   subchronic AA treatment on colon goblet cell mucin secretion. Male Wistar\n   rats were gavaged with AA for 5 days a week for 21 days. The animals were\n   divided into three groups that were gavaged with different AA concentrations\n   (0, 25, 50 mg/kg/day). Colon samples were processed for histochemical\n   (PAS-AB, HID-AB) and immunohistochemical (anti-rat MUC2 antibody) staining to\n   visualize mucins in the goblet cells. AA treatment showed an alteration in\n   mucin production and secretion in that the amount of all investigated mucin\n   types dropped. More prominent changes were detected in the upper crypt part\n   where a decreased number of goblet cell was observed. AA treatment elicited a\n   significant reduction in neutral mucins, while acidic mucins showed linearly\n   decreasing trend with respect to AA doses. Also, a linear reduction of MUC2\n   mucins was noticed. Sulfomucins were absent in the colon lower crypt in all\n   experimental groups, while in the upper crypt both sulfo- and sialomucins\n   were significantly decreased. The results of our study point to changes in\n   the synthesis, differentiation and distribution of mucins after AA treatment,\n   which can have adverse effect on colorectal health.</jats:p>","journal":"Archives of Biological Sciences","year":2016,"id":654842,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1709117,"name":"Renata Kovac","orcid":null,"position":1,"is_corresponding":false},{"id":1709118,"name":"Vesna Rajkovic","orcid":null,"position":2,"is_corresponding":false},{"id":1709119,"name":"Milica Matavulj","orcid":"0000-0001-6235-2800","position":3,"is_corresponding":false},{"id":1709116,"name":"Ivana Koledin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Subchronic exposure to acrylamide affects colon mucin secretion in juvenile wistar rats","abstract":"<jats:p>Acrylamide (AA) is an important industrial chemical worldwide. AA also forms\n   naturally in many high-carbohydrate foods (bread, French fries, coffee, etc.)\n   when they are heated. Since AA is ubiquitous in the human diet, and more than\n   one-third of the calories we take in each day come from foods with detectable\n   levels of acrylamide, the aim of this study was to determine the effect of\n   subchronic AA treatment on colon goblet cell mucin secretion. Male Wistar\n   rats were gavaged with AA for 5 days a week for 21 days. The animals were\n   divided into three groups that were gavaged with different AA concentrations\n   (0, 25, 50 mg/kg/day). Colon samples were processed for histochemical\n   (PAS-AB, HID-AB) and immunohistochemical (anti-rat MUC2 antibody) staining to\n   visualize mucins in the goblet cells. AA treatment showed an alteration in\n   mucin production and secretion in that the amount of all investigated mucin\n   types dropped. More prominent changes were detected in the upper crypt part\n   where a decreased number of goblet cell was observed. AA treatment elicited a\n   significant reduction in neutral mucins, while acidic mucins showed linearly\n   decreasing trend with respect to AA doses. Also, a linear reduction of MUC2\n   mucins was noticed. Sulfomucins were absent in the colon lower crypt in all\n   experimental groups, while in the upper crypt both sulfo- and sialomucins\n   were significantly decreased. The results of our study point to changes in\n   the synthesis, differentiation and distribution of mucins after AA treatment,\n   which can have adverse effect on colorectal health.</jats:p>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W2402812570","authors":[],"funders":[{"funder_name":"Ministry of Education, Science and Technological Development of the Republic of Serbia","grant_id":"III46001","title":null},{"funder_name":"Ministry of Education, Science and Technological Development of Republic of Serbia","grant_id":"46001","title":"Develooment and utilization of novel and traditional technologies in production of competitive food products with added valued for national and global market - CREATING WEALTH FROM THE WEALTH OF SERBIA"}],"total_grants":2,"fwci":0.401,"citation_percentile":0.69806404,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2020,"count":1},{"year":2022,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://www.doiserbia.nb.rs/ft.aspx?id=0354-46641600056K","host_type":"journal"},{"url":"http://www.doiserbia.nb.rs/ft.aspx?id=0354-46641600056K","host_type":"publisher"},{"url":"https://doi.org/10.2298/abs151015056k","host_type":"journal"},{"url":"https://doaj.org/article/e15c185e54a14e2d9e53e24e97246120","host_type":"repository"},{"url":"http://doi.org/10.2298/ABS151015056K","host_type":"repository"},{"url":"https://dx.doi.org/10.2298/abs151015056k","host_type":""}],"fields_of_study":["Potato Plant Research","Plant Pathogens and Resistance","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Mucin","Goblet cell","Crypt","Acrylamide","Mucin 2","Immunohistochemistry","Chemistry","Aberrant crypt foci","Staining","Colorectal cancer","Biology","Andrology","Endocrinology","Biochemistry","Internal medicine","Immunology","Pathology","Medicine","Epithelium","Gene expression","Cancer","Colonic disease","colon","goblet cells","QH301-705.5","mucins","Biology (General)","juvenile rats"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T08:38:43.806601Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}