{"doi":"10.2210/pdb6ifz/pdb","title":"Type III-A Csm complex, Cryo-EM structure of Csm-CTR2-ssDNA complex","abstract":null,"journal":"Worldwide Protein Data Bank","year":2018,"id":634803,"datarank":0.6830815337400812,"base_score":4.553876891600541,"endowment":4.553876891600541,"self_citation_contribution":0.6830815337400812,"citation_network_contribution":0.0,"self_endowment_contribution":0.6830815337400812,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":94,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1210169,"name":"J. Ma","orcid":"0009-0007-6699-6129","position":1,"is_corresponding":false},{"id":625727,"name":"J. Wang","orcid":"0000-0002-8202-6874","position":2,"is_corresponding":false},{"id":1366678,"name":"X. Zhang","orcid":"0009-0009-2791-1684","position":3,"is_corresponding":false},{"id":573664,"name":"Y. Wang","orcid":"0000-0001-9849-0682","position":4,"is_corresponding":false},{"id":1646598,"name":"L. You","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Type III-A Csm complex, Cryo-EM structure of Csm-CTR2-ssDNA complex","abstract":"Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, the structural features allowing target RNA-binding-dependent activation of DNA cleavage and cOA generation remain unknown. Here, we report the structure of Csm in complex with crRNA together with structures of cognate or non-cognate target RNA bound Csm complexes. We show that depending on complementarity with the 5' tag of crRNA, the 3' anti-tag region of target RNA binds at two distinct sites of the Csm complex. Importantly, the interaction between the non-complementary anti-tag region of cognate target RNA and Csm1 induces a conformational change at the Csm1 subunit that allosterically activates DNA cleavage and cOA generation. Together, our structural studies provide crucial insights into the mechanistic processes required for crRNA-meditated sequence-specific RNA cleavage, RNA target-dependent non-specific DNA cleavage, and cOA generation.","is_dataset_classified":null,"base_score":4.553876891600541,"endowment":4.553876891600541,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30503210","pmcid":null,"openalex_id":"https://openalex.org/W2903421385","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":16},{"year":2020,"count":16},{"year":2021,"count":21},{"year":2022,"count":19},{"year":2023,"count":11},{"year":2024,"count":7},{"year":2025,"count":3}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6935017","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6935017","host_type":"repository"},{"url":"https://doi.org/10.2210/pdb6ifz/pdb","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/30503210","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","RNA and protein synthesis mechanisms","Viral Infections and Immunology Research"],"mesh_terms":["RNA, Guide, CRISPR-Cas Systems","Bacterial Proteins","Deoxyribonucleases","DNA","Endoribonucleases","Models, Molecular","Ribonucleases","RNA","RNA, Bacterial","RNA, Guide, Kinetoplastida","Clustered Regularly Interspaced Short Palindromic Repeats","CRISPR-Cas Systems","CRISPR-Associated Proteins"],"keywords":["Trans-activating crRNA","RNA","Biology","CRISPR","RNase P","RNA interference","DNA","RNA silencing","RNase H","Cell biology","Computational biology","Non-coding RNA","Genetics","Cas9","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:11:24.618477Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}