{"doi":"10.21873/invivo.13108","title":"Carcinogen 4-Nitroquinoline Oxide (4-NQO) Induces Oncostatin-M (OSM) in Esophageal Cells","abstract":"Background/Aim: The earliest cellular and molecular biologic changes in the esophagus that lead to esophageal cancer were evaluated in a mouse model. We correlated numbers of senescent cells with the levels of expression of potentially carcinogenic genes in sorted side population (SP) cells containing esophageal stem cells and non-stem cells in the non-side population cells in the 4-nitroquinolone oxide (NQO)-treated esophagus. Materials and Methods: We compared stem cells with non-stem cells from the esophagus of mice treated with the chemical carcinogen 4-NQO (100 μg/ml) in drinking water. We also compared gene expression in human esophagus samples treated with 4-NQO (100 μg/ml media) to non-treated samples. We separated and quantitated the relative levels of expression of RNA using RNAseq analysis. We identified senescent cells by luciferase imaging of p16<sup>+/LUC</sup> mice and senescent cells in excised esophagus from tdTOMp16+ mice. Results: A significant increase in the levels of RNA for oncostatin-M was found in senescent cells of the esophagus from 4-NQO-treated mice and human esophagus in vitro. Conclusion: Induction of OSM in chemically-induced esophageal cancer in mice correlates with the appearance of senescent cells.","journal":"In Vivo","year":2023,"id":374375,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":228850,"name":"Michael W. Epperly","orcid":"0000-0002-6214-6887","position":1,"is_corresponding":false},{"id":674855,"name":"Renee Fisher","orcid":null,"position":2,"is_corresponding":false},{"id":674856,"name":"Donna Shields","orcid":null,"position":3,"is_corresponding":false},{"id":674375,"name":"Wen‐Chi Hou","orcid":"0000-0002-9565-7018","position":4,"is_corresponding":false},{"id":802075,"name":"Arjun Pennathur","orcid":"0000-0001-7937-7991","position":5,"is_corresponding":false},{"id":55509,"name":"James D. Luketich","orcid":"0000-0002-6578-6169","position":6,"is_corresponding":false},{"id":105025,"name":"Hong Wang","orcid":"0000-0003-0477-2908","position":7,"is_corresponding":false},{"id":110066,"name":"Joel S. Greenberger","orcid":"0000-0003-2271-7566","position":8,"is_corresponding":false},{"id":674374,"name":"Amitava Mukherjee","orcid":"0000-0001-5603-5147","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:16:11.341687Z","pmid":"36881075","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}