{"doi":"10.21873/invivo.12149","title":"Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models","abstract":"BACKGROUND/AIM: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. MATERIALS AND METHODS: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. RESULTS: The engraftment rate was 100%. CONCLUSION: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.","journal":"In Vivo","year":2020,"id":83101,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9331,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":227846,"name":"Hannah M. Hollandsworth","orcid":"0000-0002-8867-6835","position":1,"is_corresponding":false},{"id":428099,"name":"Norihiko Sugisawa","orcid":"0000-0003-1887-7268","position":2,"is_corresponding":false},{"id":428100,"name":"Jun Yamamoto","orcid":"0000-0003-2591-5023","position":3,"is_corresponding":false},{"id":428101,"name":"Yoshihiko Tashiro","orcid":"0000-0002-8574-5305","position":4,"is_corresponding":false},{"id":428102,"name":"Sachiko Inubushi","orcid":"0000-0003-0541-4942","position":5,"is_corresponding":false},{"id":428103,"name":"Kazuyuki Hamada","orcid":"0000-0003-0516-9772","position":6,"is_corresponding":false},{"id":247597,"name":"Yu Sun","orcid":"0000-0001-7121-9112","position":7,"is_corresponding":false},{"id":429217,"name":"HYEIN LIM","orcid":null,"position":8,"is_corresponding":false},{"id":429218,"name":"Siamak Amirfakhri","orcid":null,"position":9,"is_corresponding":false},{"id":429219,"name":"Filemoni Filemoni","orcid":null,"position":10,"is_corresponding":false},{"id":370841,"name":"Robert M. Hoffman","orcid":"0000-0002-0049-0041","position":11,"is_corresponding":false},{"id":399592,"name":"Michael Bouvet","orcid":"0000-0002-0086-2159","position":12,"is_corresponding":false},{"id":428098,"name":"Hiroto Nishino","orcid":"0000-0002-7377-9997","position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:54:09.383533Z","pmid":"33144418","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}