{"doi":"10.21873/invivo.12042","title":"Humanized Fluorescent Tumor-associated Glycoprotein-72 Antibody Selectively Labels Colon-cancer Liver Metastases in Orthotopic Mouse Models","abstract":"BACKGROUND/AIM: Fluorescence imaging has been shown to improve intra-operative detection of liver metastasis. The present study aimed to determine whether humanized anti-TAG-72 antibody (huCC49) conjugated to a near-infrared dye provides selective labeling of colorectal-cancer liver metastasis in orthotopic mouse models. MATERIALS AND METHODS: Humanized anti-TAG-72 (huCC49) was conjugated to IRDye800CW (huCC49-IR800). Orthotopic liver-metastasis nude-mouse models (n=5) were established with the human colon-cancer LS174T cell-line. Three weeks later, mice were administered huCC49-IR800 and intra-vital imaging was performed 48 h later. The mean tumor-to-liver ratio (TLR) was calculated. RESULTS: Intra-vital imaging demonstrated clear tumor margins with minimal liver fluorescence 48 h after administration of 50 μg huCC49-IR800 with mean TLR=7.53 (SD±2.76). CONCLUSION: Anti-TAG-72 monoclonal antibody conjugated to IRDye800 provides distinct and bright labeling of colorectal tumors in orthotopic nude-mouse models of liver metastasis. TAG-72 may be a useful target for intra-operative imaging of colorectal cancer liver metastasis in the clinic.","journal":"In Vivo","year":2020,"id":84201,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":428098,"name":"Hiroto Nishino","orcid":"0000-0002-7377-9997","position":1,"is_corresponding":false},{"id":432270,"name":"Michael A. Turner","orcid":"0000-0001-8949-3136","position":2,"is_corresponding":false},{"id":432271,"name":"Siamak Amirfakhri","orcid":"0000-0002-5534-1043","position":3,"is_corresponding":false},{"id":429219,"name":"Filemoni Filemoni","orcid":null,"position":4,"is_corresponding":false},{"id":370841,"name":"Robert M. Hoffman","orcid":"0000-0002-0049-0041","position":5,"is_corresponding":false},{"id":289782,"name":"Paul J. Yazaki","orcid":"0000-0003-2626-3303","position":6,"is_corresponding":false},{"id":399592,"name":"Michael Bouvet","orcid":"0000-0002-0086-2159","position":7,"is_corresponding":false},{"id":227846,"name":"Hannah M. Hollandsworth","orcid":"0000-0002-8867-6835","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T21:54:50.827092Z","pmid":"32871754","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}