{"doi":"10.21873/cgp.20171","title":"Duplex DNA from Sites of Helicase-Polymerase Uncoupling Links Non-B DNA Structure Formation to Replicative Stress","abstract":"BACKGROUND: Replication impediments can produce helicase-polymerase uncoupling allowing lagging strand synthesis to continue for as much as 6 kb from the site of the impediment. MATERIALS AND METHODS: We developed a cloning procedure designed to recover fragments from lagging strand near the helicase halt site. RESULTS: A total of 62% of clones from a p53-deficient tumor cell line (PC3) and 33% of the clones from a primary cell line (HPS-19I) were within 5 kb of a G-quadruplex forming sequence. Analyses of a RACK7 gene sequence, that was cloned multiple times from the PC3 line, revealed multiple deletions in region about 1 kb from the cloned region that was present in a non-B conformation. Sequences from the region formed G-quadruplex and i-motif structures under physiological conditions. CONCLUSION: Defects in components of non-B structure suppression systems (e.g. p53 helicase targeting) promote replication-linked damage selectively targeted to sequences prone to G-quadruplex and i-motif formation.","journal":"Cancer Genomics & Proteomics","year":2020,"id":86785,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8706,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":442913,"name":"Jarrod Clark","orcid":null,"position":1,"is_corresponding":false},{"id":442041,"name":"Victoria Bedell","orcid":"0000-0002-6706-1807","position":2,"is_corresponding":false},{"id":442042,"name":"Joyce Murata‐Collins","orcid":"0000-0001-6568-005X","position":3,"is_corresponding":false},{"id":289777,"name":"Marianna Martella","orcid":"0000-0003-2389-3975","position":4,"is_corresponding":false},{"id":442043,"name":"Flavia Pichiorri","orcid":"0009-0006-8429-9397","position":5,"is_corresponding":false},{"id":442914,"name":"Emily F. Warner","orcid":null,"position":6,"is_corresponding":false},{"id":442044,"name":"Mahmoud A. S. Abdelhamid","orcid":"0000-0002-1247-9915","position":7,"is_corresponding":false},{"id":442045,"name":"Zoë A. E. Waller","orcid":"0000-0001-8538-0484","position":8,"is_corresponding":false},{"id":442046,"name":"Steven S. Smith","orcid":"0000-0003-0222-3403","position":9,"is_corresponding":false},{"id":442912,"name":"Camille Amparo","orcid":null,"position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-18T21:59:03.706190Z","pmid":"32108033","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}