{"doi":"10.21873/anticanres.15412","title":"Activity in MCF-7 Estrogen-sensitive Breast Cancer Cells of Capsicodendrin from<i>Cinnamosma fragrans</i>","abstract":"Background/Aim: Effect of capsicodendrin on the NF-κB pathway was studied in MCF-7 cancer cells. Materials and Methods: The transcription factor assay was used to screen for NF-κB activity. The effect on IKKβ, ICAM-1, and caspase-7 were studied using western blot. Caspase-1 was studied using Promega Caspase-Glo<sup>®</sup> assay. Reactive oxygen species (ROS) were detected using the fluorescent probe DCFH-DA. The potentiometric dye JC-1 was used to assess mitochondrial membrane potential (ΔΨm) and the cell cycle was examined using a fluorescence-activated cell sorter. Results: NF-κB p65 inhibitory effect was IC<sub>50</sub>=8.6 μM and cytotoxic activity was IC<sub>50</sub>=7.5 μM. The upstream IKK and the downstream ICAM-1 were down-regulated. Sub G<sub>1</sub>-phase population increased to 81% after 12 h of treatment with capsicodendrin (10 μM) and there was no loss of ΔΨM. Conclusion: Increased levels of intracellular ROS promoted activity of caspase-1 and induced cell death in MCF-7 cells. Capsicodendrin may be a future anticancer agent that prevents the progression of metastatic breast cancer.","journal":"Anticancer Research","year":2021,"id":201859,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":781323,"name":"Nathan P. Mirtallo Ezzone","orcid":"0000-0002-1757-2801","position":1,"is_corresponding":false},{"id":781951,"name":"L. HARINANTENAINA RAKOTONDRAIBE","orcid":null,"position":2,"is_corresponding":false},{"id":360864,"name":"Esperanza J. Carcache de Blanco","orcid":"0000-0002-9519-3292","position":3,"is_corresponding":false},{"id":781950,"name":"Ulyana Muñoz Acuña","orcid":null,"position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-18T23:51:05.955461Z","pmid":"34848447","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}