{"doi":"10.21769/bioprotoc.4892","title":"Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M","abstract":"and heparin), how to remove the solubility tags with TEV protease and recover untagged proteins of interest, and how to overcome commonly encountered challenges in obtaining high yield of each protein. Key features • This protocol builds upon purification methods developed by Patel lab (Ramachandran et al., 2017) and others with greater detail than previously published works. • The protocol requires several days to complete as various steps are designed to be performed overnight. • The recombinantly purified proteins have been successfully used for in vitro transcription experiments, allowing for finer control of experimental components in a minimalistic system.","journal":"BIO-PROTOCOL","year":2023,"id":363216,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9305,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":900779,"name":"Sean D. Reardon","orcid":"0000-0002-6439-4593","position":1,"is_corresponding":false},{"id":1117700,"name":"Jubilee H Munozvilla-Cabellon","orcid":null,"position":2,"is_corresponding":false},{"id":364377,"name":"Jiayu Shen","orcid":"0000-0001-8447-9378","position":3,"is_corresponding":false},{"id":364381,"name":"Smita S. Patel","orcid":"0000-0002-2523-4933","position":4,"is_corresponding":false},{"id":900780,"name":"Tatiana V. Mishanina","orcid":"0000-0001-7400-3501","position":5,"is_corresponding":false},{"id":1117239,"name":"An Hsieh","orcid":"0000-0003-3552-1132","position":0,"is_corresponding":true}],"reference_count":11,"raw_metadata":null,"created_at":"2026-07-19T01:14:28.054440Z","pmid":"38094251","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}