{"doi":"10.2174/1389202923666220527112929","title":"A Global Analysis of Alternative Splicing of Dichocarpum Medicinal\nPlants, Ranunculales","abstract":"<jats:sec>\n<jats:title>Background:</jats:title>\n<jats:p>The multiple isoforms are often generated from a single gene via alternative splicing (AS) in plants, and the functional diversity of plant genome is significantly increased. Despite well studied gene functions, the specific functions of isoforms are little known, therefore the accurate prediction of isoform functions is exceedingly wanted.</jats:p>\n</jats:sec>\n<jats:sec>\n<jats:title>Methods:</jats:title>\n<jats:p>Here we perform the first global analysis of AS of Dichocarpum, a medicinal genus of Ranunculales, by utilizing full-length transcriptome datasets of five Chinese endemic Dichocarpum taxa. Multiple softwares were used to identify AS events, the gene function was annotated based on seven databases, and the protein coding sequence of each AS isoform was translated into an amino acid sequence. The self-developed software DIFFUSE was used to predict the functions of AS isoforms.</jats:p>\n</jats:sec>\n<jats:sec>\n<jats:title>Results:</jats:title>\n<jats:p>Among 8,485 genes with AS events, the genes with two isoforms were the most (6,038), followed by those with three isoforms and four isoforms. Retained intron (RI, 551) was predominant among 1,037 AS events, and alternative 3' splice sites and alternative 5' splice sites were second. The software DIFFUSE was effective in predicting functions of Dichocarpum isoforms, which have not been unearthed. When compared with the sequence alignment-based database annotations, DIFFUSE performed better in differentiating isoform functions. The DIFFUSE predictions on the terms GO:0003677 (DNA binding) and GO: 0010333 (terpene synthase activity) agreed with biological features of transcript isoforms.</jats:p>\n</jats:sec>\n<jats:sec>\n<jats:title>Conclusion:</jats:title>\n<jats:p>Numerous AS events were for the first time identified from full-length transcriptome datasets of five Dichocarpum taxa, and functions of AS isoforms were successfully predicted by the self-developed software DIFFUSE. The global analysis of Dichocarpum AS events and predicting isoform functions can help understand metabolic regulations of medicinal taxa and their pharmaceutical explorations.</jats:p>\n</jats:sec>","journal":"Current Genomics","year":2022,"id":20610,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":1,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":67.5,"fair_percentile":96.41600703605981,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":108705,"name":"Tao Jiang","orcid":"0009-0007-6524-5382","position":1,"is_corresponding":false},{"id":12579,"name":"Hao Chen","orcid":"0009-0001-6480-7976","position":2,"is_corresponding":false},{"id":135629,"name":"Pei-Gen Xiao","orcid":null,"position":3,"is_corresponding":false},{"id":135628,"name":"Da-Cheng Hao","orcid":"0000-0001-6589-4023","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36777007","pmcid":"PMC9878827","openalex_id":"https://openalex.org/W4281686626","authors":[],"funders":[{"funder_name":"Scientific Research Funds Project of the Liaoning Education Department","grant_id":"JDL2019012","title":null},{"funder_name":"China Scholarship Council","grant_id":"202108210156","title":null},{"funder_name":"US National Institute of Health","grant_id":"1R01NS125018","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2018YFC0910404","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01NS125018-02","title":"RNA Regulatory Networks in Neuronal Cell Type Diversity and Function"}],"total_grants":5,"fwci":0.0866,"citation_percentile":0.4157115,"influential_citations":0,"citation_trend":[{"year":2025,"count":1}],"oa_status":"green","license":"cc-by-nc","oa_locations":[{"url":"https://escholarship.org/uc/item/5pf943f3","host_type":"repository"},{"url":"https://escholarship.org/content/qt5pf943f3/qt5pf943f3.pdf?t=s7i6yr","host_type":"GREEN"},{"url":"https://escholarship.org/uc/item/5pf943f3","host_type":"repository"},{"url":"https://www.eurekaselect.com/article/download?doi=10.2174/1389202923666220527112929","host_type":"publisher"},{"url":"https://www.eurekaselect.com/205336/article","host_type":"publisher"},{"url":"https://doi.org/10.2174/1389202923666220527112929","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36777007","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9878827","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9878827","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9878827?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.2174/1389202923666220527112929","host_type":""},{"url":"https://escholarship.org/content/qt5pf943f3/qt5pf943f3.pdf","host_type":""},{"url":"https://doi.org/https://doi.org/10.2174/1389202923666220527112929","host_type":""}],"fields_of_study":["Plant biochemistry and biosynthesis","Plant Reproductive Biology","Ziziphus Jujuba Studies and Applications","Medicine","Biology","Environmental Science","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Traditional medicine","Computational biology","Biology","Medicine","DNA sequence","Gene expression profile","Alternative splicing","Deep Learning","Dichocarpum","Isoform Function","570","610","Genetics & Genomics"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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