{"doi":"10.2144/btn-2023-0051","title":"Multiparameter Screen Optimizes Immunoprecipitation","abstract":"Immunoprecipitation (IP) coupled with mass spectrometry effectively maps protein–protein interactions when genome-wide, affinity-tagged cell collections are used. Such studies have recorded significant portions of the compositions of physiological protein complexes, providing draft ‘interactomes’; yet many constituents of protein complexes still remain uncharted. This gap exists partly because high-throughput approaches cannot optimize each IP. A key challenge for IP optimization is stabilizing in vivo interactions during the transfer from cells to test tubes; failure to do so leads to the loss of genuine interactions during the IP and subsequent failure to detect. Our high-content screening method explores the relationship between in vitro chemical conditions and IP outcomes, enabling rapid empirical optimization of conditions for capturing target macromolecular assemblies.","journal":"BioTechniques","year":2024,"id":459263,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1286042,"name":"Leila J Saba","orcid":"0000-0002-5513-4335","position":1,"is_corresponding":false},{"id":318216,"name":"Hua Jiang","orcid":"0000-0002-9917-2683","position":2,"is_corresponding":false},{"id":1286456,"name":"Omar Rosas Bringas","orcid":null,"position":3,"is_corresponding":false},{"id":484423,"name":"Mehrnoosh Oghbaie","orcid":"0000-0002-8225-3436","position":4,"is_corresponding":false},{"id":1284691,"name":"Luciano Di Stéfano","orcid":null,"position":5,"is_corresponding":false},{"id":480194,"name":"Vadim Sherman","orcid":null,"position":6,"is_corresponding":false},{"id":463098,"name":"John LaCava","orcid":"0000-0002-6307-7713","position":7,"is_corresponding":false},{"id":1284272,"name":"Shaoshuai Xie","orcid":"0000-0002-4391-4676","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-19T02:03:55.280882Z","pmid":"38425263","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}