{"doi":"10.2144/btn-2020-0153","title":"A Comparison of DNA/RNA Extraction Protocols for High-Throughput Sequencing of Microbial Communities","abstract":"One goal of microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods the authors previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, the authors compared the relative performance of two total nucleic acid extraction protocols with the authors' previously benchmarked protocol. The authors included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here the authors present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection and well-to-well contamination between these protocols.","journal":"BioTechniques","year":2021,"id":156159,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9324,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":499161,"name":"Clarisse Marotz","orcid":"0000-0002-1520-8854","position":1,"is_corresponding":false},{"id":225242,"name":"Pedro Belda‐Ferre","orcid":"0000-0001-6532-1161","position":2,"is_corresponding":false},{"id":106555,"name":"Cameron Martino","orcid":"0000-0001-9334-1258","position":3,"is_corresponding":false},{"id":347187,"name":"Stephen Wandro","orcid":"0000-0001-5514-0244","position":4,"is_corresponding":false},{"id":654775,"name":"Mehrbod Estaki","orcid":"0000-0003-4556-8444","position":5,"is_corresponding":false},{"id":270109,"name":"Rodolfo A. Salido","orcid":"0000-0003-0631-2817","position":6,"is_corresponding":false},{"id":235440,"name":"Carolina S. Carpenter","orcid":"0000-0002-5716-3068","position":7,"is_corresponding":false},{"id":268001,"name":"Lívia S. Zaramela","orcid":"0000-0002-1065-3799","position":8,"is_corresponding":false},{"id":558716,"name":"Jeremiah J. Minich","orcid":"0000-0002-7202-965X","position":9,"is_corresponding":false},{"id":555815,"name":"MacKenzie Bryant","orcid":"0000-0003-0749-2995","position":10,"is_corresponding":false},{"id":270110,"name":"Karenina Sanders","orcid":"0000-0002-6793-5770","position":11,"is_corresponding":false},{"id":660151,"name":"Serena Fraraccio","orcid":"0000-0002-5097-8176","position":12,"is_corresponding":false},{"id":3537,"name":"Gail Ackermann","orcid":"0000-0002-3901-4931","position":13,"is_corresponding":false},{"id":270112,"name":"Gregory Humphrey","orcid":"0000-0003-3930-1663","position":14,"is_corresponding":false},{"id":3540,"name":"Austin D. Swafford","orcid":"0000-0001-5655-8300","position":15,"is_corresponding":false},{"id":660152,"name":"Sandrine Miller‐Montgomery","orcid":"0000-0003-4969-9505","position":16,"is_corresponding":false},{"id":3554,"name":"Rob Knight","orcid":"0000-0002-0975-9019","position":17,"is_corresponding":false},{"id":555814,"name":"Justin P. Shaffer","orcid":"0000-0002-9371-6336","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T23:44:08.368845Z","pmid":"33512248","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}