{"doi":"10.2131/jts.38.783","title":"Di-(2-ethylhexyl)-phthalate reduces MyoD and myogenin expression and inhibits myogenic differentiation in C2C12 cells","abstract":null,"journal":"The Journal of Toxicological Sciences","year":2013,"id":635250,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1648040,"name":"Hsiao-Ting Hung","orcid":null,"position":1,"is_corresponding":false},{"id":1648042,"name":"Tsan-Ju Chen","orcid":null,"position":2,"is_corresponding":false},{"id":1648044,"name":"Hui-Shan Hung","orcid":null,"position":3,"is_corresponding":false},{"id":1648046,"name":"Dean-Chuan Wang","orcid":null,"position":4,"is_corresponding":false},{"id":1648038,"name":"Shun-Sheng Chen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Di-(2-ethylhexyl)-phthalate reduces MyoD and myogenin expression and inhibits myogenic differentiation in C2C12 cells","abstract":"The purpose of this study was to investigate the effects of di-(2-ethylhexyl) phthalate (DEHP) treatment on MyoD and myogenin expression and myotube formation in the murine C2C12 cells. Myogenic differentiation is principally regulated by activities of myogenic regulatory factors, such as MyoD and myogenin, leading the elongation and fusion of mononucleated myoblasts into multinucleated myotubes. In the present study, myogenic differentiation of C2C12 cells was induced by serum deprivation with medium containing vehicle or DEHP (10, 100, 1,000 μg/ml) for 5 days. Using 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay clearly demonstrated cell viability was not affected by DEHP at any given dose. At the dose of 1,000 μg/ml DEHP, the elongation of multinucleated myotubes, and the percent of nuclei incorporated into myosin heavy chain (MyHC)-stained myotubes were markedly reduced. In addition, immunoblotting revealed expression of muscle specific marker MyHC, as well as myogenic regulatory factors MyoD and myogenin, were reduced in DEHP-treated myotubes during myogenic differentiation. Taken together, the results showed that DEHP may impair myogenic differentiation through repression of myogenic regulatory factors, such as MyoD and myogenin, resulting in a reduction of MyHC expression. This in vitro study suggests that DEHP may be an environmental risk factor for myogenesis.","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24067726","pmcid":null,"openalex_id":"https://openalex.org/W1976822897","authors":[],"funders":[],"total_grants":0,"fwci":0.2228,"citation_percentile":0.60005007,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2017,"count":2},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/jts/38/5/38_783/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/jts/38/5/38_783/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.2131/jts.38.783","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24067726","host_type":"repository"}],"fields_of_study":["Effects and risks of endocrine disrupting chemicals","Additive Manufacturing and 3D Printing Technologies","biodegradable polymer synthesis and properties","Animals","Cell Differentiation","Cell Survival","Cells, Cultured","Diethylhexyl Phthalate","Down-Regulation","Endocrine Disruptors","Gene Expression","Gene Expression Regulation, Developmental","Mice","MyoD Protein","Myoblasts","Myogenin","Plasticizers"],"mesh_terms":["Animals","Cell Differentiation","Cell Survival","Cells, Cultured","Diethylhexyl Phthalate","Plasticizers","Down-Regulation","Gene Expression","MyoD Protein","Myogenin","Gene Expression Regulation, Developmental","Myoblasts","Mice","Endocrine Disruptors"],"keywords":["MyoD","Myogenin","Myogenesis","C2C12","MYF5","Myocyte","Myosin","Multinucleate","Cellular differentiation","Myogenic regulatory factors","MyoD Protein","Chemistry","Biology","Molecular biology","Endocrinology","Cell biology","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:50:45.606565Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}