{"doi":"10.21273/jashs.121.5.810","title":"Susceptibility of Common and Tepary Beans to Agrobacterium spp. Strains and Improvement of Agrobacterium-mediated Transformation Using Microprojectile Bombardment","abstract":"<jats:p>\n                    To develop an efficient protocol for\n                    <jats:italic>Agrobacterium</jats:italic>\n                    -mediated transformation of common bean (\n                    <jats:italic>Phaseolus vulgaris</jats:italic>\n                    L.) and tepary bean (\n                    <jats:italic>P. acutifolius</jats:italic>\n                    A. Gray), we have tested the susceptibility of six genotypes to eight\n                    <jats:italic>Agrobacterium tumefaciens</jats:italic>\n                    and two\n                    <jats:italic>A. rhizogenes</jats:italic>\n                    strains. The virulence of the\n                    <jats:italic>Agrobacterium</jats:italic>\n                    strains was shown to be genotype dependent. In general, the tumors observed on common bean cultivars were larger than those observed on tepary bean cultivars. The\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    AT8196 and Ach5 strains and the\n                    <jats:italic>A. rhizogenes</jats:italic>\n                    8196 strain induced the best responses in all genotypes tested. Polymerase chain reaction (PCR) analysis confirmed the presence of T-DNA in tumors derived from inoculation with three\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    strains in common beans. Apical meristems of\n                    <jats:italic>P. vulgaris</jats:italic>\n                    cv. Jalo were bombarded with tungsten microprojectiles and then inoculated with an\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    wild-type strain (Ach5). One month later, the explants showed a high frequency of tumor formation (50% to 70%). Similarly, when bombarded meristems were inoculated with an\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    disarmed strain (LBA4404/p35SGUSINT), 44% of them showed substantial sectors of GUS activity, suggesting the expression of introduced gene. The bombardment/\n                    <jats:italic>Agrobacterium</jats:italic>\n                    system appears to be a promising method to stably transform bean through the regeneration of plants directly from transformed apical meristems.\n                  </jats:p>","journal":"Journal of the American Society for Horticultural Science","year":1996,"id":688588,"datarank":0.5533319181170905,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"self_citation_contribution":0.5533319181170905,"citation_network_contribution":0.0,"self_endowment_contribution":0.5533319181170905,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1798936,"name":"Francisco J. Lima Aragão","orcid":null,"position":1,"is_corresponding":false},{"id":1798939,"name":"Sílvia Rossi","orcid":null,"position":2,"is_corresponding":false},{"id":1798941,"name":"Diva Maria A. Dusi","orcid":null,"position":3,"is_corresponding":false},{"id":1798943,"name":"Leila M. Gomes Barros","orcid":null,"position":4,"is_corresponding":false},{"id":1798945,"name":"Elíbio L. Rech","orcid":null,"position":5,"is_corresponding":false},{"id":1798933,"name":"Ana Cristina M. Brasileiro","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Susceptibility of Common and Tepary Beans to Agrobacterium spp. Strains and Improvement of Agrobacterium-mediated Transformation Using Microprojectile Bombardment","abstract":"<jats:p>\n                    To develop an efficient protocol for\n                    <jats:italic>Agrobacterium</jats:italic>\n                    -mediated transformation of common bean (\n                    <jats:italic>Phaseolus vulgaris</jats:italic>\n                    L.) and tepary bean (\n                    <jats:italic>P. acutifolius</jats:italic>\n                    A. Gray), we have tested the susceptibility of six genotypes to eight\n                    <jats:italic>Agrobacterium tumefaciens</jats:italic>\n                    and two\n                    <jats:italic>A. rhizogenes</jats:italic>\n                    strains. The virulence of the\n                    <jats:italic>Agrobacterium</jats:italic>\n                    strains was shown to be genotype dependent. In general, the tumors observed on common bean cultivars were larger than those observed on tepary bean cultivars. The\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    AT8196 and Ach5 strains and the\n                    <jats:italic>A. rhizogenes</jats:italic>\n                    8196 strain induced the best responses in all genotypes tested. Polymerase chain reaction (PCR) analysis confirmed the presence of T-DNA in tumors derived from inoculation with three\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    strains in common beans. Apical meristems of\n                    <jats:italic>P. vulgaris</jats:italic>\n                    cv. Jalo were bombarded with tungsten microprojectiles and then inoculated with an\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    wild-type strain (Ach5). One month later, the explants showed a high frequency of tumor formation (50% to 70%). Similarly, when bombarded meristems were inoculated with an\n                    <jats:italic>A. tumefaciens</jats:italic>\n                    disarmed strain (LBA4404/p35SGUSINT), 44% of them showed substantial sectors of GUS activity, suggesting the expression of introduced gene. The bombardment/\n                    <jats:italic>Agrobacterium</jats:italic>\n                    system appears to be a promising method to stably transform bean through the regeneration of plants directly from transformed apical meristems.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W2169891497","authors":[],"funders":[],"total_grants":0,"fwci":0.4098,"citation_percentile":0.61671811,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":1},{"year":2019,"count":1},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://journals.ashs.org/downloadpdf/journals/jashs/121/5/article-p810.pdf","host_type":"journal"},{"url":"https://journals.ashs.org/downloadpdf/journals/jashs/121/5/article-p810.pdf","host_type":"publisher"},{"url":"https://journals.ashs.org/view/journals/jashs/121/5/article-p810.xml","host_type":"publisher"},{"url":"https://journals.ashs.org/downloadpdf/journals/jashs/121/5/article-p810.xml","host_type":"publisher"},{"url":"https://doi.org/10.21273/jashs.121.5.810","host_type":"journal"},{"url":"http://americanae.aecid.es/americanae/es/registros/registro.do?tipoRegistro=MTD&idBib=2103950","host_type":"repository"},{"url":"http://www.alice.cnptia.embrapa.br/alice/handle/doc/170805","host_type":"repository"}],"fields_of_study":["Plant tissue culture and regeneration","Plant pathogens and resistance mechanisms","Transgenic Plants and Applications"],"mesh_terms":[],"keywords":["Agrobacterium tumefaciens","Agrobacterium","Biology","Phaseolus","Transformation (genetics)","Rhizobiaceae","Meristem","Inoculation","Explant culture","Botany","Horticulture","Shoot","Bacteria","Gene","Genetics","Symbiosis","In vitro"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T17:43:23.797738Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}