{"doi":"10.21203/rs.3.rs-3474151/v1","title":"Concordance between two monoclonal antibody (mAb)-based enzyme-linked immunosorbent assays for the measurement of cysticercal antigen levels in sera of pigs experimentally infected with Taenia solium and Taenia hydatigena","abstract":"Abstract Background Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158 / B60 antigen-detection ELISA (Ag-ELISA) has high levels of sensitivity and specificity in human neurocysticercosis (94% and 99% according to the manufacturer), although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay performance is affected by its frequent cross-reaction with Taenia hydatigena . Our group has produced 21 anti- T. solium monoclonal antibodies (mAbs) reacting against antigens of the whole cyst, vesicular fluid, and secretory/excretory products, identifying TsW8 / TsW5 as the most promising pair of mAbs for an Ag-ELISA. Methods We report the use of our TsW8 / TsW5 Ag-ELISA for the measurement of parasite antigen levels in two panels of sera from pigs experimentally infected with T. solium (n = 26) and T. hydatigena (n = 12) from day 0 to day 90 post-infection (PI) and assessed the concordance of our assay with the reference B158 / B60 Ag-ELISA using Bland-Altman (BA) plots and Lin’s concordance coefficients (LCC). Results Our TsW8 / TsW5 Ag-ELISA measured antigen levels efficiently during the course of T. solium cysticercosis infections in pigs with a strong agreement with the reference B158 / B60 Ag-ELISA in almost all paired log-transformed optical density (OD) values. The limits of agreement (LoA) in the BA plot at days 0, 28, and 90 PI were 92.3%, 100% and 100%, respectively, and high concordance between assays was also found (LCC: 0.69, 0.92, and 0.96, respectively, all P < 0.001). In pigs infected with T. hydatigena , almost all paired log-transformed ODs were also within the LoA, whereas concordance between assays was low or moderate at days 0 and 28 PI, (LCC: 0.24 and 0.88), but higher at day 90 PI (LCC: 0.98, P < 0.001). Conclusions / significance: The TsW8 / TsW5 Ag-ELISA recognizes antigens in pigs with T. solium cysticercosis and is highly concordant with the B158 / B60 Ag-ELISA. However, its diagnostic use is hampered by cross-reactions with T. hydatigena , as in other mAb antigen detection assays.","journal":"Research Square","year":2023,"id":413465,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":939742,"name":"Luz Toribio","orcid":null,"position":1,"is_corresponding":false},{"id":1195100,"name":"Sara Garrido","orcid":null,"position":2,"is_corresponding":false},{"id":939313,"name":"Nancy Chile","orcid":"0000-0003-1851-8118","position":3,"is_corresponding":false},{"id":1074530,"name":"María Teresa Rugeles López","orcid":"0000-0002-8389-4139","position":4,"is_corresponding":false},{"id":939314,"name":"Luis A. Gomez‐Puerta","orcid":"0000-0002-7909-979X","position":5,"is_corresponding":false},{"id":1195101,"name":"Miguel Muro","orcid":null,"position":6,"is_corresponding":false},{"id":304253,"name":"Robert H. Gilman","orcid":"0000-0002-9037-0712","position":7,"is_corresponding":false},{"id":1079048,"name":"Yesenia Castillo","orcid":"0000-0003-1971-1702","position":8,"is_corresponding":false},{"id":728646,"name":"Pierre Dorny","orcid":"0000-0001-5381-7987","position":9,"is_corresponding":false},{"id":467888,"name":"Seth E. O’Neal","orcid":"0000-0002-4782-3368","position":10,"is_corresponding":false},{"id":467890,"name":"Armando E. González","orcid":"0000-0003-1909-1873","position":11,"is_corresponding":false},{"id":455137,"name":"Héctor H. Garcı́a","orcid":"0000-0002-4633-6269","position":12,"is_corresponding":false},{"id":467884,"name":"Gianfranco Arroyo","orcid":"0000-0001-8743-5012","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T01:21:57.641539Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}