{"doi":"10.21203/rs.2.16083/v1","title":"User Manual for Tomography-Guided 3D Reconstruction of Subcellular Structures (TYGRESS)","abstract":"<title>Abstract</title>\n        <p>Recent advances in cryo-electron microscopy (cryo-EM) are paving the way to determining isolated three-dimensional (3D) macromolecular structures at near-atomic resolution using single-particle cryo-electron microscopy (SP-cryo-EM). However, determining the subcellular structures in intact cells and organelles<italic> </italic>using cryo-electron tomography (cryo-ET) and subtomogram averaging, another cryo-EM technique, with comparable resolution remains a challenge. Current methodologies can only reach a resolution of several nanometers in most samples studied. Here, we introduce a new hybrid method, called Tomography-Guided 3D Reconstruction of Subcellular Structures (TYGRESS) that is able to achieve structural determination of subcellular structures within their natural crowded environment with nanometer-resolution by combining the advantages of cryo-ET and SP-cryo-EM.</p>","journal":null,"year":null,"id":627458,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":543960,"name":"Kangkang Song","orcid":"0000-0002-5499-5221","position":1,"is_corresponding":false},{"id":1065483,"name":"Xiaofeng Fu","orcid":"0000-0001-6194-0408","position":2,"is_corresponding":false},{"id":322060,"name":"Xiaochu Lou","orcid":null,"position":3,"is_corresponding":false},{"id":291544,"name":"Nikolaus Grigorieff","orcid":"0000-0002-1506-909X","position":4,"is_corresponding":false},{"id":320834,"name":"Daniela Nicastro","orcid":"0000-0002-0122-7173","position":5,"is_corresponding":false},{"id":340673,"name":"Zhiguo Shang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-08-04T17:34:27.567065Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}