{"doi":"10.2108/zsj.29.333","title":"Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis","abstract":null,"journal":"Zoological Science","year":2012,"id":588886,"datarank":0.5389979661278737,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.27023404574366533,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.27023404574366533,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":5,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1506672,"name":"Sakiko Shimizu","orcid":null,"position":1,"is_corresponding":false},{"id":1506673,"name":"Taketoshi Kiya","orcid":null,"position":2,"is_corresponding":false},{"id":167825,"name":"Kazuei Mita","orcid":null,"position":3,"is_corresponding":false},{"id":1506674,"name":"Masafumi Iwami","orcid":null,"position":4,"is_corresponding":false},{"id":263068,"name":"Anuradha Roy","orcid":"0000-0002-1057-1443","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis","abstract":"The insect brain secretes prothoracicotropic hormone (PTTH), which stimulates the prothoracic gland to synthesize ecdysone. The active metabolite of ecdysone, 20-hydroxyecdysone (20E), works through ecdysone receptor (EcR) and ultraspiracle (USP) to initiate molting and metamorphosis by regulating downstream genes. Previously, we found that EcR was expressed in the PTTH-producing neurosecretory cells (PTPCs) in larval brain of the silkworm Bombyx mori, suggesting that PTPCs function as the master cells of development under the regulation of 20E. To gain a better understanding of the molecular mechanism of the 20E control of PTPCs, we performed a comprehensive screening of genes induced by 20E using DNA microarray with brains of day-2 fifth instar silkworm larvae. Forty-one genes showed greater than twofold changes caused by artificial application of 20E. A subsequent semiquantitative screening identified ten genes upregulated by 20E, four of which were novel or not previously identified as 20E-response genes. Developmental profiling determined that two genes, UP4 and UP5, were correlated with the endogenous ecdysteroid titer. Whole-mount in situ hybridization showed exclusive expression of these two genes in two pairs of cells in the larval brain in response to 20E-induction, suggesting that the cells are PTPCs. BLAST searches revealed that UP4 and UP5 are Bombyx homologs of vrille and tarsal-less, respectively. The present study identifies 20E-induced genes that may be involved in the ecdysone signal hierarchies underlying pupal-adult development and/or the 20E regulation of PTPCs.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22559968","pmcid":null,"openalex_id":"https://openalex.org/W2066894264","authors":[],"funders":[],"total_grants":0,"fwci":0.1231,"citation_percentile":0.43402104,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2023,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://kanazawa-u.repo.nii.ac.jp/records/10492","host_type":"repository"},{"url":"https://kanazawa-u.repo.nii.ac.jp/records/10492","host_type":"repository"},{"url":"http://www.bioone.org/doi/pdf/10.2108/zsj.29.333","host_type":"publisher"},{"url":"https://doi.org/10.2108/zsj.29.333","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22559968","host_type":"repository"},{"url":"http://www.bioone.org/doi/10.2108/zsj.29.333","host_type":"repository"},{"url":"https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=10492","host_type":"repository"}],"fields_of_study":["Neurobiology and Insect Physiology Research","Physiological and biochemical adaptations","Insect and Arachnid Ecology and Behavior","Animals","Bombyx","Brain","Ecdysterone","Gene Expression Profiling","Gene Expression Regulation","Larva","Oligonucleotide Array Sequence Analysis"],"mesh_terms":["Animals","Brain","Ecdysterone","Gene Expression Regulation","Larva","Bombyx","Oligonucleotide Array Sequence Analysis","Gene Expression Profiling"],"keywords":["Biology","Prothoracic gland","Ecdysone","Bombyx mori","20-Hydroxyecdysone","Ecdysone receptor","Ecdysteroid","Gene","Bombyx","Cell biology","Molecular biology","Nuclear receptor","Transcription factor","Genetics","Endocrinology","Hormone"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T00:29:27.244042Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}