{"doi":"10.20944/preprints202501.1266.v1","title":"Lateral Ventricular Neural Stem Cells (LV NSCs) Regulate Neural Circuitry Activity Through GABAergic Signaling","abstract":"Recent studies have demonstrated that circuit activation in vivo can regulate lateral ventricular neural stem cells (LV NSCs), although the underlying molecular and cellular mechanisms are not yet fully understood. Here we investigated the role of GABAergic signaling in the bidirectional interaction between LV NSCs and the anterior cingulate cortex-subependymal-ChAT+ (ACC-subep-ChAT+) circuit. We found that monoamine oxidase B (MAOB), a key enzyme involved in GABA synthesis, is expressed in LV NSCs, and that activation of the ACC-subep-ChAT+ circuit can modulate MAOB activity. Additionally, LV NSCs express lrrc8d, a core component of volume-regulated anion channels, and Slc6a1, the gene encoding GABA transporter-1 (GAT-1). LRRC8D and GAT-1 can regulate neural circuit activity through GABA signaling to regulate proliferation of LV NSCs. Collectively, these findings underscore the pivotal role of GABA signaling in NSC function, mediated by MAOB-driven GABA synthesis, LRRC8D-regulated GABA transport, and GAT-1-facilitated GABA reuptake, which together regulate neural circuit activation and influence NSC proliferation dynamics.","journal":"Preprints.org","year":2025,"id":560529,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9563,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":351424,"name":"Henry H. Yin","orcid":"0000-0003-1546-6850","position":1,"is_corresponding":false},{"id":864665,"name":"Moawiah M. Naffaa","orcid":"0000-0003-0451-5901","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:55:42.883572Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}