{"doi":"10.20944/preprints202501.0490.v1","title":"A Cost-Effective and Easy to Assemble 3D Human Microchannel-Blood-Brain Barrier Model and Its Application in Tumor Metastasis","abstract":"By utilizing PDMS, collagen hydrogel and a cell line for human cerebral microvascular endothelial cells, we generated a 3D microchannel-BBB model under physiological flows. This 3D BBB has a circular shaped cross-section and a diameter of ~100m, which can properly mimic the cerebral microvessel responsible for material exchange between the circulating blood and brain tissue. The permeability of the 3D microchannel-BBB to a small molecule (sodium fluorescein with molecular weight 376) and that to a large molecule (Dex-70k) are the same as those of rat cerebral microvessels. This 3D BBB model can replicate the response to a plasma protein, orosomucoid, a cytokine, VEGF, and an enzyme, heparinase III, in either rat cerebral or mesenteric microvessesels in terms of permeability and glycocalyx (heparan sulfate). It can also replicate the adhesion of a breast cancer cell, MDA-MB-231, in rat mesenteric microvessels with no treatment and with treatments with VEGF, orosomucoid and heparinase III. Because of difficulties in accessing human cerebral microvessels, this inexpensive and easy to assemble 3D human BBB model can be applied to investigate the BBB modulating mechanisms in health and in disease and to develop therapeutic interventions targeting tumor metastasis to the brain.","journal":"Preprints.org","year":2025,"id":560118,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.915,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1462272,"name":"Yunfei Li","orcid":"0000-0002-0441-0643","position":1,"is_corresponding":false},{"id":309328,"name":"Bingmei M. Fu","orcid":"0000-0001-9343-5895","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:55:42.883572Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}