{"doi":"10.18609/cgti.2022.190","title":"IsoTag™AAV: an innovative, scalable & non-chromatographic method for streamlined AAV manufacturing","abstract":"Demand for gene therapies capable of treating previously inaccessible targets has risen precipitously in the past decade. Adeno-associated viruses (AAVs) are the preferred vector for gene delivery because of their favorable safety profile and tissue tropism, but they have significant manufacturing challenges, with end-to-end yields as low as 10–30%. To combat these low yields, we developed IsoTag™AAV, a novel purification technology for AAV that is a departure from the chromatographic paradigm in downstream processing. This proprietary technology uses a self-scaffolding recombinant protein reagent that can improve manufacturing yields. It enables purification by cost-effective and scalable filtration processes and improves product quality with minimal optimization. Herein, we describe the development of IsoTag™AAV, provide a head-to-head comparison to industry-leading affinity chromatography (evaluation carried out through a joint research project with Capsida Biotherapeutics), and demonstrate how it can reduce cost of goods for a clinical AAV program by 25%.","journal":"Cell and Gene Therapy Insights","year":2022,"id":288449,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":969253,"name":"J. B. Jones","orcid":"0000-0002-0773-2007","position":1,"is_corresponding":false},{"id":969668,"name":"Sophia Petraki","orcid":null,"position":2,"is_corresponding":false},{"id":969669,"name":"Melissa Callander","orcid":null,"position":3,"is_corresponding":false},{"id":969670,"name":"Shaleen Shrestha","orcid":null,"position":4,"is_corresponding":false},{"id":969671,"name":"Emily Springfield","orcid":null,"position":5,"is_corresponding":false},{"id":969672,"name":"Laura Adamson","orcid":null,"position":6,"is_corresponding":false},{"id":231603,"name":"Ashutosh Chilkoti","orcid":"0000-0002-1569-2228","position":7,"is_corresponding":false},{"id":969673,"name":"Michael J Dzuricky","orcid":null,"position":8,"is_corresponding":false},{"id":670818,"name":"Kelli M. Luginbuhl","orcid":"0000-0002-6713-0331","position":9,"is_corresponding":false},{"id":969667,"name":"Jennifer Haley","orcid":null,"position":0,"is_corresponding":true}],"reference_count":17,"raw_metadata":null,"created_at":"2026-07-19T00:30:14.968626Z","pmid":"37712070","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}